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Quantikine human atx immunoassay

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The Quantikine human ATX immunoassay is a quantitative sandwich enzyme immunoassay designed to measure the concentration of human autotaxin (ATX) in cell culture supernates, serum, and plasma. It is a laboratory tool used for the quantitative determination of the target analyte.

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Lab products found in correlation

Slt rainbow plate reader, by Tecan (1 mentions)

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Quantikine Human Immunoassay (17 citations) Quantikine Immunoassays (15 citations) Quantikines (4 citations) Quantikine Human (2 citations)

2 protocols using quantikine human atx immunoassay

1

Determination of Serum ATX Levels

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Serum ATX levels were determined with the Quantikine human ATX immunoassay (R&D Systems, Minneapolis, Minnesota), according to the recommendation of the manufacturer. All measurements were performed on a Tecan SLT Rainbow Plate Reader (Tecan, Männedorf, Switzerland).
Pleli T., Martin D., Kronenberger B., Brunner F., Köberle V., Grammatikos G., Farnik H., Martinez Y., Finkelmeier F., Labocha S., Ferreirós N., Zeuzem S., Piiper A, & Waidmann O. (2014). Serum Autotaxin Is a Parameter for the Severity of Liver Cirrhosis and Overall Survival in Patients with Liver Cirrhosis – A Prospective Cohort Study. PLoS ONE, 9(7), e103532.
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2

Quantitative Enzyme Immunoassay for ATX

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All serum and CSF samples were brought to room temperature before use in an enzyme-linked immunosorbent assay. Serum and CSF ATX levels were determined with a Quantikine human ATX immunoassay (R&D Systems, Minneapolis, MN) following the manufacturer’s procedure. Quantification was performed with a Multi-Spectrophotometer Viento Microplate Reader (Sumitomo Dainippon Pharma Co, Osaka, Japan). The person performing the ATX assays at NHOKMC was unaware of the treatment status of the patients from whom the samples were obtained.
Itagaki K., Takebayashi M., Abe H., Shibasaki C., Kajitani N., Okada-Tsuchioka M., Hattori K., Yoshida S., Kunugi H, & Yamawaki S. (2019). Reduced Serum and Cerebrospinal Fluid Levels of Autotaxin in Major Depressive Disorder. International Journal of Neuropsychopharmacology, 22(4), 261-269.
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