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Mhcc97l

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MHCC97L is a cell line derived from human hepatocellular carcinoma. It is used for research and experimental purposes in the field of oncology and liver disease.

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Lab products found in correlation

Mhcc 97h, by Shanghai Cell Bank (10 mentions) Hepg2, by Shanghai Cell Bank (7 mentions) Smmc 7721, by Shanghai Cell Bank (7 mentions) Hep3b, by Shanghai Cell Bank (5 mentions) Sk hep 1, by Shanghai Cell Bank (4 mentions) Bel 7402, by Shanghai Cell Bank (4 mentions) Hcclm3, by Shanghai Cell Bank (3 mentions) Plc prf 5, by Shanghai Cell Bank (3 mentions) Hepg2, by Thermo Fisher Scientific (2 mentions) Qsg 7701, by Shanghai Cell Bank (2 mentions) Mhcc lm3, by Shanghai Cell Bank (2 mentions) Bel 7404, by Shanghai Cell Bank (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Sk hep1, by Thermo Fisher Scientific (1 mentions) Qgy 7703, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Qgy 7701, by Thermo Fisher Scientific (1 mentions) Qsg 7701, by Thermo Fisher Scientific (1 mentions) Hl 7702, by Thermo Fisher Scientific (1 mentions) Interferin transfection reagent, by Polyplus Transfection (1 mentions)

14 protocols using mhcc97l

1

Culturing Human Hepatocellular Carcinoma Cells

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The normal hepatocyte LO2 cells, as well as the commercially available HCC cell lines Huh-7, HepG2, HCC-LM3, SMMC-7721 and MHCC-97L, were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Home-made patient-derived HCC cell lines PDC-26#, PDC-14#, PDC-12#, PDC-9#, PDC-23#, PDC-10# and PDC-11# were established as described above. All of these cells were cultured in Dulbecco’s modified eagle medium (DMEM) supplemented with 10% (v/v) FBS and 1% penicillin-streptomycin at 37 °C in a humidified incubator under 5% CO2 condition.
Cai J., Sun X., Guo H., Qu X., Huang H., Yu C., Wu H., Gao Y., Kong X, & Xia Q. (2020). Non-metabolic role of UCK2 links EGFR-AKT pathway activation to metastasis enhancement in hepatocellular carcinoma. Oncogenesis, 9(12), 103.
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2

Comparative Analysis of Hepatocellular Carcinoma Cell Lines

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Human hepatocellular carcinoma cell lines (HepG2, QGY7701, QGY7703, QSG7701, Huh-7, MHcc97L, MHcc97H and SK-HEP-1), one mouse hepatocellular carcinoma Hepal-6 cell line and immortalized normal human liver HL7702 and L-02 cell lines were used in this study. HepG2, QGY7701, Hepal-6 and QGY7703 cell lines were obtained from Shanghai Maternal and Child Health Hospital (Shanghai, China). L-02, MHcc97L, MHcc97H, QSG7701, Huh-7 and SK-HEP-1 cell lines were purchased from the Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China). The HL7702 cell line was obtained from the Chinese Academy of Science (Kunming, China). HepG2, QSG7701, L-02, HL7702 and Hepal-6 cells were maintained in RPMI-1640 medium (Gibco-BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco-BRL). QGY7701, QGY7703, Huh-7, SK-HEP-1, MHcc97L and MHcc97H cells were maintained in Dulbecco’s modified Eagle’s medium (Gibco-BRL) supplemented with 10% FBS. All cell lines were cultured at 37°C in a humidified incubator with an atmosphere of 5% CO2.
LIU J., SHU Y., ZHANG Q., LIU B., XIA J., QIU M., MIAO H., LI M, & ZHU R. (2014). Dihydromyricetin induces apoptosis and inhibits proliferation in hepatocellular carcinoma cells. Oncology Letters, 8(4), 1645-1651.
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3

Hepatocyte and Hepatocellular Carcinoma Cell Culture Protocol

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The normal-type hepatocyte QSG7701 and HL7702 cells, as well as the HCC cell lines Huh-7, SNU-475, HepG2, Hep3B, PLC/PRF/5, SMC7721, MHCC-97L, and MHCC-97H, were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained at 37°C in an atmosphere containing 5% CO2 in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum. Lentivirus productions for stable cell line construction were completed by the Genechem Company (Shanghai, China) and used according to the manufacturer’s instructions. For RNA interfering, cells were transfected with 100 nM small interfering (si)RNAs by INTERFERin transfection reagent (409–10; Polyplus, New York, NY) according to manufacturer’s instructions. siRNAs were purchased from the Biotend Company (Shanghai, China). For more information, see the Supplementary Methods.
Lin X.M., Hu L., Gu J., Wang R.Y., Li L., Tang J., Zhang B.H., Yan X.Z., Zhu Y.J., Hu C.L., Zhou W.P., Li S., Liu J.F., Gonzalez F.J., Wu M.C., Wang H.Y, & Chen L. (2017). Choline Kinase α Mediates Interactions Between the Epidermal Growth Factor Receptor and Mechanistic Target of Rapamycin Complex 2 in Hepatocellular Carcinoma Cells to Promote Drug Resistance and Xenograft Tumor Progression. Gastroenterology, 152(5), 1187-1202.
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4

Culturing HCC Cell Lines

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HCC cell line BLC-2, Hep3B and Huh7 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). MHCC97L, MHCC97H and SUHC-1 were purchased from the Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China), and HCCLM3 were purchased from China Center for Type Culture Collection (Wuhan, China). All the cell lines were cultured using DMEM adding 10% fetal bovine (FBS; HyClone, UT, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, St Louis, MO, USA).
Feng S., Liu J., Hailiang L., Wen J., Zhao Y., Li X., Lu G., Gao P, & Zeng X. (2021). Amplification of RAD54B promotes progression of hepatocellular carcinoma via activating the Wnt/β-catenin signaling. Translational Oncology, 14(8), 101124.
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5

Cohort Study of HCC Tissue and Cells

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Two independent cohorts composed of 216 patients with HCC were enrolled in this study (cohort 1, n = 136; cohort 2, n = 80). Paired HCC tissues and adjacent liver tissues were collected from patients who underwent liver resection at the First Affiliated Hospital of Harbin Medical University between January 2008 and August 2013. All patients provided written informed consent and this study was approved by the Research Ethics Committee of the First Affiliated Hospital of Harbin Medical University. Follow-up data were collected from patients after hepatic resection to monitor and assess the overall rate of cancer metastasis and recurrence. A normal liver cell line (L02) and several HCC cell lines (Huh7, HCCLM3, SK-Hep-1, MHCC97H, MHCC97L, SMMC7721, and HepG2) were purchased from Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China).
Meng F., Zhang S., Song R., Liu Y., Wang J., Liang Y., Wang J., Han J., Song X., Lu Z., Yang G., Pan S., Li X., Liu Y., Zhou F., Wang Y., Cui Y., Zhang B., Ma K., Zhang C., Sun Y., Xin M, & Liu L. (2019). NCAPG2 overexpression promotes hepatocellular carcinoma proliferation and metastasis through activating the STAT3 and NF-κB/miR-188-3p pathways. EBioMedicine, 44, 237-249.
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6

Culturing Human Liver Cancer Cell Lines

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The human HCC cell lines (Huh7, Hep3B, HepG2, MHCC-LM3, MHCC-97L, and MHCC-97H) were purchased from Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China). They were cultured in DMEM medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) and grown at 37 °C in a 5% CO2 environment. The content of anagliptin used in the study was 20 µM.
Cui X., Yun X., Sun M., Li R., Lyu X., Lao Y., Qin X, & Yu W. (2022). HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility. Hepatology International, 17(2), 377-392.
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7

Culturing Diverse Liver Cancer Cell Lines

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Bel-7402, Bel-7404, HepG2, SMMC-7721, QGY-7703, Hep3B, Huh7, MHCC97L, MHCC97H, PLC/PRF/5 and HCCLM3 cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences in China. LO2 and HEK293T cells were obtained from the Cancer Research Institute of Southern Medical University in Guangzhou, China. Cells were propagated in DMEM supplemented with 10% (v/v) fetal bovine serum (Biowest, Loire Valley, France) at 37 °C in a humidified atmosphere of 5% CO2 in an incubator.
Lin X., Zuo S., Luo R., Li Y., Yu G., Zou Y., Zhou Y., Liu Z., Liu Y., Hu Y., Xie Y., Fang W, & Liu Z. (2019). HBX-induced miR-5188 impairs FOXO1 to stimulate β-catenin nuclear translocation and promotes tumor stemness in hepatocellular carcinoma. Theranostics, 9(25), 7583-7598.
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8

Establishing HCC Cell Line Cultures

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Five hepatocellular carcinoma (HCC) cell lines (HepG3B, Huh-7, THLE-3, MHCC97L and MHCC97H) were purchased from Shanghai Cell Bank of Chinese Academy of Sciences, and culture conditions were as follows: 10% fetal bovine serum and RPMI1640/DMEM mixed medium (1:1), 37 °C, and 5% CO2 incubator.
Wu L., Liao W., Wang X., Zhao Y., Pang J., Chen Y., Yang H, & He Y. (2021). Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis. Bioengineered, 12(2), 10302-10318.
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9

Cell Culture of Liver and Kidney Lines

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Non-malignant liver cells (LO2), the human embryonic kidney cell line 293 T, and human hepatoma cell lines (SMMC-7721, Bel-7402, MHCC-97 L, and MHCC-97H) were obtained from Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All of the cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Hyclone, Logan, UT, USA) supplemented with 10 % fetal bovine serum (Invitrogen, Grand Island, NY, USA) and 1 % penicillin G and streptomycin in 37 °C humidified air containing 5 % CO2.
Lu X., Zhou C., Li R., Liang Z., Zhai W., Zhao L, & Zhang S. (2016). Critical role for the long non-coding RNA AFAP1-AS1 in the proliferation and metastasis of hepatocellular carcinoma. Tumour Biology, 37(7), 9699-9707.
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10

Prostate and Liver Cancer Cell Response to HUVEC-CM

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The human prostate cancer cell DU-145 was a generous gift from Dr. Hong, Chen (Zhejiang University, China). HUVEC cell lines and Human hepatocellular carcinoma cell lines MHCC-LM3, MHCC-97L, Bel-7402 were purchased from Shanghai Cell Bank, Chinese Academy of Sciences. HUVEC cells were maintained in RPMI-1640 (Gibco) containing 10% FBS (fetal bovine serum) (Sigma-Aldrich) and incubated at 37°C in a 5% CO2 water-saturated environment. Conditioned medium of HUVEC cells (HUVEC-CM) was collected as previously described 12 . RPMI-1640 medium supplemented with 10% FBS severed as the control medium. MHCC-LM3, MHCC-97L, Bel-7402 and DU-145 cells were respectively incubated in the HUVEC-CM for 21 days (n=3). MHCC-LM3 and MHCC-97L were subcultured once a week at a ratio of 1:2. Bel-7402, DU-145 and HUVEC were subcultured once a week at a ratio of 1:3 or 1:5.
Ding S.M., Lu A.L., Lu J.F., Chen X.L., Edoo M.I., Zhou L., Xie H.Y., Zheng S.S, & Li Q.Y. (2020). Macrovascular Endothelial Cells Enhance the Motility of Liver Cancer Cells by Up-regulation of MMP-3, Activation of Integrin/FAK Signaling Pathway and Induction of Non-classical Epithelial-mesenchymal Transition. Journal of Cancer, 11(8), 2044-2059.
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