Hba1c assay kit

The HbA1c test, is an important blood test that gives a good indication of controlled diabetes condition. For this test, heart-collected blood sample was utilized to measure rat HbA1c levels in HbA1c assay kit (Crystal chem, cat number: 80300).

For 7-week experiment, the db/db mice with 23.7 ± 2.5 mM blood glucose were chosen, and the once daily EXE doses were the same as those described above. The BGL and body weight were measured before administration at predetermined time points. The mice had free access to water and standard chow during the whole experiment.
After the 7-week administration, the db/db mice were fasting for 12 h, and then the blood sample was collected from the eye ground vein. The HbA_1c concentration in the whole blood sample was analyzed using HbA_1c assay kit (Crystal Chem Inc., Elk Grove Village, Illinois, USA). The insulin and C-peptide concentrations in the plasma of the sample were analyzed using the corresponding Elisa kit (Crystal Chem Inc.).

Glycated hemoglobin (HbA_1c) has been validated as a reliable marker to measure glycemic control in mice (Feng et al., 2019 (link); Han et al., 2008 (link); Dan et al., 1997 (link)). Whole blood HbA_1c and plasma levels of TAG and prolactin were measured. HbA_1c was measured in whole undiluted blood collected in EDTA tubes using a commercial HbA_1c assay kit (Crystal Chem, Elk Grove Village, IL, USA). Plasma TAG was measured by preparing a 1:5 dilution using the Cayman Chemical kit. Plasma prolactin was measured in a 1:20 dilution of plasma using a commercially available ELISA kit (ab100736, Abcam, MA, USA). All assays were performed following the manufacturers’ instructions.