Urban pm srm 1648a

Manufactured by Merck Group

Sourced in United States

Urban PM (SRM 1648a) is a reference material developed by the National Institute of Standards and Technology (NIST) for the analysis of particulate matter in urban air. It is composed of particulate matter collected from the atmosphere in an urban area. The material is intended to be used for the calibration and verification of analytical methods for the determination of the chemical composition of particulate matter in urban air samples.

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SRM 1648a (3 citations)

2 protocols using urban pm srm 1648a

Molecular Mechanisms of Oxidative Stress

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Anti-GAPDH, anti-phospho-JNK1/2, anti-β-actin, anti-caspase-3, anti-caspase-7, anti-caspase-9, anti-poly(ADP-ribose) polymerase (PARP), anti-Bax, anti-Bad, anti-Bcl-2, anti-cytochrome c, anti-Akt, and anti-COX IV antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). SP600125, Z-VAD-FMK, MitoTEMPO, surfactin, and urban PM (SRM 1648a) were purchased from Sigma (St. Louis, MO, USA). N-acetyl-L-cysteine (NAC) and diphenyleneiodonium chloride (DPI) were taken from Biomol (Plymouth Meeting, PA).

Vo T.T., Liu J.F., Wu C.Z., Lin W.N., Chen Y.L, & Lee I.T. (2020). Surfactin from Bacillus subtilis induces apoptosis in human oral squamous cell carcinoma through ROS-regulated mitochondrial pathway. Journal of Cancer, 11(24), 7253-7263.

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Investigating Intracellular Oxidative Stress

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Urban PM (SRM 1648a) and tricarbonyldichlororuthenium (II) dimer (CORM-2) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The inactive variant iCORM-2 was generated through the inactivation of CORM-2 by replacing carbonyl groups with DMSO, as described in a previous study [32 (link)]. Apocynin (APO), N-acetyl-L-cysteine (NAC), diphenyleneiodonium chloride (DPI), and helenalin (HLN) were obtained from Sigma-Aldrich (St. Louis, MO, USA); whereas BCECF-AM (2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester) was taken from Invitrogen (CA, USA).

Vo T.T., Hsu C.Y., Wee Y., Chen Y.L., Cheng H.C., Wu C.Z., Lin W.N, & Lee I.T. (2021). Carbon Monoxide-Releasing Molecule-2 Ameliorates Particulate Matter-Induced Aorta Inflammation via Toll-Like Receptor/NADPH Oxidase/ROS/NF-κB/IL-6 Inhibition. Oxidative Medicine and Cellular Longevity, 2021, 2855042.

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