Unless otherwise stated, all chemicals were obtained from Sigma Chemical Co., St. Louis, MO, USA. Mouse monoclonal anti-rat transferrin receptor 1 (TfR1) and goat anti-mouse IgG-Alexa 488 were purchased from Invitrogen, Carlsbad, CA, USA; rabbit polyclonal anti-rat divalent metal transporter 1 (DMT1, SLC11A2) and rabbit polyclonal anti-ferritin-heavy-chain (Ft-L) from Proteintech, Chicago, IL, USA; rabbit polyclonal anti-ferritin-hight-vhain (Ft-H) from Bioworld Technology Inc., Louis Park, MN, USA; and rabbit polyclonal anti-mouse ferroportin 1 (Fpn1) from Novus Biologicals, Littleton, CO, USA. Goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibody was bought from Li-Cor, Lincoln, NE, USA; TRIzol reagent from Life Technologies, Carlsbad, CA, USA; and AevertAid First Strand cDNA Synthesis Kit and BCA Protein Assay Kit both from Thermo Scientific, Waltham, MA, USA. Microtubule associated protein 2 (MAP2) and astrocyte marker glial fibrillary acidic protein (GFAP) were obtained from Chemicon International, Inc. Temecula, CA, USA; the in situ cell death detection kit, TMR red from USA Roche, Nutley, NJ, USA. The Health Department of Hong Kong and Shanghai Government and the Animal Research Ethics Committee of The Chinese University of Hong Kong and Fudan University approved the experimental procedures of this study.
Goat anti rabbit or anti mouse irdye 800 cw secondary antibody
Manufactured by LI COR
Sourced in United States
The Goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibody is a fluorescently labeled secondary antibody used for detection in Western blotting, immunohistochemistry, and other immunoassays. The antibody is labeled with the near-infrared dye IRDye 800 CW, which can be detected using compatible near-infrared imaging systems.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (6 mentions)
Aevertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (5 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (5 mentions)
Rabbit polyclonal anti mouse fpn1, by Novus Biologicals (3 mentions)
Mouse monoclonal anti β actin, by Merck Group (3 mentions)
Faststart universal sybr green master and lightcycler96, by Roche (3 mentions)
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
Mouse anti human tfr1, by Thermo Fisher Scientific (2 mentions)
Bca protein assay kit and protein ripa lysis buffer, by Beyotime (2 mentions)
Goat anti mouse igg alexa 488, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rabbit anti gpx4, by Abcam (1 mentions)
Rabbit anti apoe, by Abcam (1 mentions)
Rabbit anti map2, by Abcam (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Rabbit anti irp2, by Abcam (1 mentions)
Rabbit anti ho 1, by Abcam (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Faststart universal sybr green master, by Roche (1 mentions)
Lightcycler 96, by Roche (1 mentions)
7 protocols using goat anti rabbit or anti mouse irdye 800 cw secondary antibody
1
Iron Homeostasis Regulation in Neurodegenerative Disorders
2
Transferrin Receptor Regulation in Iron Homeostasis
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Unless otherwise stated, all chemicals were obtained from the Sigma Chemical Company, St. Louis, MO, USA. Mouse monoclonal anti-rat transferrin receptor 1 (TfR1) was purchased from Invitrogen, Carlsbad, CA, USA; rabbit polyclonal anti-rat divalent metal transporter 1 (DMT1, SLC11A2) and rabbit polyclonal anti ferritin-light-chain (Ft-L) from Proteintech, Chicago, IL, USA; rabbit polyclonal anti ferritin-heavy-chain (Ft-H) from Bioworld Technology Inc., Louis Park, MN, USA; rabbit polyclonal anti-mouse ferroportin 1 (Fpn1) from Novus Biologicals, Littleton, CO, USA; mouse anti-transferrin (Tf) and anti-ceruloplasmin (CP) from Alpha Diagnostic International Company, San Antonio, TX, USA. Goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibody was purchased from Li-Cor, Lincoln, NE, USA; TRIzol reagent from Life Technologies, Carlsbad, CA, USA; and RevertAid First Strand cDNA Synthesis Kit and BCA Protein Assay Kit both from Thermo Scientific, Waltham, MA, USA.
3
Immunoblotting Analysis of Cellular Iron Homeostasis
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Unless otherwise stated, all chemicals including mouse monoclonal anti-β-actin were obtained from the Sigma Chemical Company, St. Louis, MO, USA. Mouse anti-human TfR1, Alexa Fluor 488 goat anti-rabbit IgG, TRIzol reagent, RPMI-1640 medium and fetal bovine serum were purchased from Invitrogen Life Technologies, Carlsbad, CA, USA; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, USA; rabbit polyclonal anti-FTL (ferritin light chain) from Proteintech, Chicago, IL, USA; rabbit polyclonal anti-FTH (ferritin heavy chain) from Bioworld Technology Inc., Louis Park, MN, USA; rabbit anti-MAP2, rabbit anti-Aβ42, rabbit anti-HO1, rabbit anti-Gpx4, rabbit anti-ApoE, rabbit anti-IRP1 (iron regulatory protein 1) and rabbit anti-IRP2 (iron regulatory protein 2) from Abcam, Cambridge, MA, USA; and goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibody from LI-COR Biosciences, Lincoln, Nebraska, USA. AevertAid First Strand cDNA Synthesis Kit and BCA protein assay kits were bought from Thermo Scientific, Waltham, MA, USA; Faststart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, USA; and protein RIPA lysis buffer from the Beyotime Institute of Biotechnology, Haimen, JS, China. All solutions were prepared fresh, prior to each assay.
4
Molecular Mechanisms of Iron Regulation
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Unless otherwise stated, all chemicals were obtained from the Sigma Chemical Company, St. Louis, MO, USA. Mouse monoclonal anti-rat TfR1 was purchased from Invitrogen, Carlsbad, CA, USA; rabbit polyclonal anti-Fpn1 from Novus Biologicals, Littleton, CO, USA; mouse anti-p-JAK2 (phospho-Janus Kinase 2) from Cell Signaling Technology, Boston, USA; TRIzol reagent from Life Technologies, Carlsbad, CA, USA; and AevertAid First Strand cDNA Synthesis Kit from Thermo Scientific, Waltham, MA, USA. Rabbit polyclonal anti-Ft-Land anti-DMT1 were purchased from Protein-tech, Chicago, IL, USA; rabbit polyclonal anti-CBS, HIF-2α and EPO antibodies from Abcam, Inc., Cambridge, UK; rabbit polyclonal anti-Ft-H from Bioworld Technology Inc., Louis Park, MN, USA; goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibody from LI-COR Bio Sciences, Lincoln, NE, USA; FastStart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, USA; and BCA protein Assay kit and protein RIPA lysis buffer from Beyotime Institute of Biotechnology, Haimen, JS, China.
5
Iron Homeostasis Regulation in Mouse Macrophages
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Unless otherwise stated, all chemicals were obtained from the Sigma Chemical Company, St. Louis, MO, United States. Mouse anti-rat TfR1 (transferrin receptor 1) and fetal bovine serum (FBS) were purchased from Invitrogen, Carlsbad, CA, United States; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, United States; rabbit polyclonal anti-Ft-L and anti-DMT1 (divalent metal transporter 1, SLC11A2) from Protein-tech, Chicago, IL, United States; and rabbit polyclonal anti-phospho-STAT3 (Tyr705) and mouse monoclonal anti-STAT3 both from Cell Signaling Technology, Inc., Danvers, MA, United States. Recombined mouse IL-6 protein was obtained from Gene Script, Piscataway, NJ, United States; IL-6 enzyme-linked immunosorbent assay (ELISA) kits from R&D Systems, Minneapolis, MN, United States; goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibodies from LI-COR Bio Sciences, Lincoln, NE, United States; TRIzol reagent from Life Technologies, Carlsbad, CA, United States; AevertAid First Strand cDNA Synthesis Kit from Thermo Scientific, Waltham, MA, United States; FastStart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, United States; and BCA protein Assay kit and protein RIPA lysis buffer from Beyotime Institute of Biotechnology, Haimen, JS, China.
6
Exploring Iron Regulation in Mouse Cell Culture
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Unless otherwise stated, all chemicals including mouse monoclonal anti-β-actin were obtained from Sigma-Aldrich Chemical Co., St. Louis, MO, U.S.A. The recombinant mouse HGF protein was obtained from R&D Systems, Minneapolis, MN, U.S.A.; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, U.S.A.; mouse anti-human TfR1 from Invitrogen Life technologies, Carlsbad, CA, U.S.A.; rabbit polyclonal anti-ferritin light chain (Ft-L) and anti-DMT1 from Proteintech, Chicago, IL, U.S.A.; and rabbit monoclonal anti-IRP1 and anti-IRP2 were from Abcam, San Francisco, CA, U.S.A. Goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibodies were bought from LI-COR Bio Sciences, Lincoln, NE, U.S.A.; the bicinchoninic acid (BCA) protein assay kit and AevertAid First Strand cDNA Synthesis Kit from Thermo Fisher Scientific, Waltham, MA, U.S.A.; the TRIzol reagent from Life technologies, Carlsbad, CA, U.S.A.; FastStart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, U.S.A.; protein RIPA lysis buffer from Beyotime Institute of Biotechnology, Haimen, JS, China. Recombinant HGF protein was dissolved in phosphate buffer saline (PBS) and diluted to 1–120 ng/ml before use.
7
Antibody-mediated Iron Regulation in Cells
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Unless otherwise stated, all chemicals, including MTT and mouse monoclonal anti-β-actin, were obtained from the Sigma Chemical Company, St. Louis, MO, USA. The mouse anti-rat TfR1 antibody was purchased from Invitrogen, Carlsbad, CA, USA, and the rabbit polyclonal anti-mouse Fpn1 antibody from Chemicon International, Temecula, CA, USA. The rabbit anti-rat DMT1 + IRE and DMT1 - IRE polyclonal antibodies were bought from the Alpha Diagnostic International Company, San Antonio, TX, USA, and goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibodies from LI-COR Biosciences, Lincoln, NE, USA. Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F-12 was obtained from GIBCO, Grand Island, NY, USA and BSA from the Calbiochem-Novabiochem Corporation, San Diego, CA, USA. The scintillation cocktail and tubes were obtained from the Beckman Coulter Company, Fullerton, CA, USA. All experimental protocols were performed according to the Animal Management Rules of the Ministry of Health of China, and approved by the Animal Ethics Committees of Fudan University and The Chinese University of Hong Kong.
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