α mem without phenol red

Manufactured by Thermo Fisher Scientific

α-MEM without phenol red is a powdered cell culture medium formulation that does not contain the pH indicator phenol red. This medium supports the growth and maintenance of various cell types in vitro.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Millex gp 0.22 μm filter, by Merck Group (1 mentions) Amicon ultra 4 centrifugal ultrafiltration tubes nmwl 3kda, by Merck Group (1 mentions) Ethidium homodimer 1, by Thermo Fisher Scientific (1 mentions) Dextran coated charcoal, by Merck Group (1 mentions) β estradiol, by MP Biomedicals (1 mentions)

Close spelling variants of this product

α-MEM (2804 citations) Phenol red (557 citations) Phenol (68 citations) MEM without phenol red (5 citations) α-MEM complete medium without phenol red (2 citations)

3 protocols using α mem without phenol red

Conditioned Medium Cytokine Analysis

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Fifth-passage RIFs were cultured in α-MEM (without phenol red; Gibco) without FBS for 24 hours. Supernatants were collected and filtered with MILLEX-GP 0.22-μm filters (Merck Millipore Ltd. Co., Cork, Munster, Ireland) to remove cell debris and concentrated to 1% volume with Amicon Ultra-4 centrifugal ultrafiltration tubes (NMWL 3KDa) (Merck Millipore Ltd. Co.) for use as conditioned medium (CM).
The concentrations of IL-1β, IL-6, TNF-α and activated TGF-β1 in CM was detected by ELISA kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions and assessed by densitometry analysis as previously described^{56 (link)}.

Jin J., Tao J., Gu X., Yu Z., Wang R., Zuo G., Li Q., Lv X, & Miao D. (2017). P16INK4a Deletion Ameliorated Renal Tubulointerstitial Injury in a Stress-induced Premature Senescence Model of Bmi-1 Deficiency. Scientific Reports, 7, 7502.

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Irisin Mediated Oxidative Stress Response

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MLO-Y4 cells were seeded in type-I collagen coated 96 well plate (3000 cells/well) in 1% FBS, 1% CS, α-MEM without phenol red (Gibco, 41061–029) on day 0. The medium was aspirated and 1% FBS, 1% CS, α-MEM without phenol red containing the indicated concentration of irisin was added to the wells. After 24 hours incubation, 0.5% FBS, 0.5% CS, α-MEM without phenol red containing the indicated concentration of irisin and 0.3mM H₂O₂ were added and the cells were incubated for 4 hours. The cells were stained with 2μM ethidium Homodimer-1 (ThermoFisher Scientific, E1169) to detect dead cells. The cell images were taken using Nikon Eclipse TE300 inverted fluorescence microscope with a Photometrics Coolsnap EZ cooled CCD camera and analyzed using ImageJ. Percentage of cell death was calculated as EthD-1 positive cells divided by the total number of cells stained with 5µg/mL Hoechst 33342 (ThermoFisher Scientific, H3570) as a nuclear counterstain (Kitase et al., 2018 (link)).

Kim H., Wrann C.D., Jedrychowski M., Vidoni S., Kitase Y., Nagano K., Zhou C., Chou J., Parkman V.J., Novick S.J., Strutzenberg T.S., Pascal B.D., Le P.T., Brooks D.J., Roche A.M., Gerber K.K., Mattheis L., Chen W., Tu H., Bouxsein M.L., Griffin P.R., Baron R., Rosen C.J., Bonewald L.F, & Spiegelman B.M. (2018). Irisin Mediates Effects on Bone and Fat via αV Integrin Receptors. Cell, 175(7), 1756-1768.e17.

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Estrogen Modulates BMMSC Autophagy

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BMMSCs were cultured in α-MEM without phenol red (Life Technologies-Gibco) containing 20% FBS. Hormones in FBS were previously removed by dextran coated charcoal (Sigma, USA). β-estradiol (MP, USA) was added to the media at a range of dosages (0, 0.1, 1, 10, 100, 1000 nM). BMMSCs were collected 48 h later to measure autophagy activity.

Qi M., Zhang L., Ma Y., Shuai Y., Li L., Luo K., Liu W, & Jin Y. (2017). Autophagy Maintains the Function of Bone Marrow Mesenchymal Stem Cells to Prevent Estrogen Deficiency-Induced Osteoporosis. Theranostics, 7(18), 4498-4516.

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