IPTT‐300 transponders (BioMedic Data Systems) were implanted subcutaneously into the backs of mice and rats using sterile trocars provided by the manufacturer. Temperature monitoring was initiated 2 weeks after implantation to avoid measuring wound healing‐related temperature variations. The subcutaneous temperature was recorded every 30 min for 24 h periods using a DAS‐7006/7 s reader system (BioMedic Data Systems).
Iptt 300 transponder
Manufactured by Avidity Science
Sourced in United States
The IPTT-300 is a transponder device used for the identification and monitoring of animals in laboratory settings. It functions by transmitting a unique identification code that can be detected by compatible readers. The core purpose of the IPTT-300 is to provide a reliable method for tracking and identifying individual animals within a controlled environment.
Automatically generated - may contain errors
Lab products found in correlation
Das 8007 p reader, by Avidity Science (3 mentions)
Das 6001 smart probe, by Avidity Science (1 mentions)
Tegaderm, by 3M (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Mini mitter, by Philips (1 mentions)
Uridine, by Merck Group (1 mentions)
Microct scanner, by GE Healthcare (1 mentions)
B6 cg tg k18 ace2 2prlmn j, by Jackson ImmunoResearch (1 mentions)
Inhaled isoflurane, by Baxter (1 mentions)
Male sprague dawley rats, by Inotiv (1 mentions)
12 protocols using iptt 300 transponder
1
Subcutaneous Temperature Monitoring in Rodents
2
Diurnal Rhythm in Thermoneutral Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Temperature measurements were carried out on 2-month–1.5-year- (5.5-month median) old male and female Id2−/− mice and WT littermates, housed individually in a climate-controlled room set to either normal (21°C ± 1°C) or thermoneutral (30°C ± 1°C) temperature. Body temperature sampling was conducted at 3 h intervals over the 24 h LD cycle. For thermoneutral conditions measurement, all WT and Id2−/− mice used in the studies were allowed to acclimate to thermoneutral temperature for 1 week before temperature measurement. Core body temperature was measured using subcutaneously surgically implanted telemetric transmitters positioned proximal to the iBAT (IPTT 300 transponders, Bio Medic Data Systems, Seaford, DE) following isoflurane anesthetization [3 (link)]. After a week of recuperation, core temperatures were recorded over a 24 h period.
3
Sensitization and Challenge Protocol for Ovalbumin-Induced Hypothermia
4
Melanocortin-Induced Thermoregulatory Responses
5
Ferret Model for Tuberculosis Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spayed female or neutered male ferrets (3-8 months old) were obtained (Triple F Farms, Sayre, PA). After acclimatization, ferrets were implanted subcutaneously with IPTT-300 transponders (BioMedic Data Systems, Seaford, DE) for identification and routine body temperature monitoring. Prior to M. tuberculosis infection, blood, nasal wash, and throat swabs were collected. Animals were pair-housed in individually ventilated stainless steel cages (Allentown, Inc., Allentown, PA) and provided with ferret High Density Diet 5L14 (LabDiet, St. Louis, MO) and fresh water ad libitum. These studies were conducted in accordance with recommendations from the Guide for the Care and Use of Laboratory Animals and within a program accredited by the Association for Assessment and Accreditation of Laboratory Animal Care, International (AAALAC-I). All animal experiments were performed with approval of the Institutional Animal Care and Use Committee at the University of Georgia (NIH Animal Welfare Assurance Number: A3437-01). Infected ferret activity level and respiratory distress (e.g. coughing) were assessed daily, temperatures were measured minimally once per week, and weight was assessed weekly.
6
Guinea Pig Model of Coxiella burnetii Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the day of infection, animals were sedated by isoflurane using an anesthetic vaporizer with activated charcoal adsorption filters (VetEquip Inc, cat. n. 901801 and 931401) and an IPTT-300 transponder (BioMedic Data Systems) was implanted subcutaneously above the shoulder of each animal in a longitudinal orientation using a large bore needle. Four guinea pigs per group were infected with 105 GE of C. burnetii in United States Pharmacopeia-grade saline via intraperitoneal injection. Four negative control animals were mock infected with United States Pharmacopeia-grade saline for each experiment. Body weight, body temperature, and behavioral and clinical changes were recorded daily. Body temperatures were collected using a DAS-8007-P reader (BioMedic Data Systems) and a temperature of ≥39.5°C was defined as fever [37 –39 ]. Body weight index (BWI) was calculated as defined by Russell-Lodrigue et al. [37 ].
7
C. burnetii Infection Model in Guinea Pigs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the day of infection, animals were sedated by isoflurane inhalation using an anesthetic vaporizer with activated charcoal adsorption filters (VetEquip Inc, cat. n. 901801 and 931401) and an IPTT-300 transponder (BioMedic Data Systems) was implanted subcutaneously above the shoulder of each animal in a longitudinal orientation using a large bore needle. Four guinea pigs per group were infected with 106 GE of C. burnetii in USP-grade saline via intraperitoneal injection. Negative control animals were mock infected with USP-grade saline for each experiment. Body weights, body temperatures, and any behavioral/clinical changes were recorded daily following infections. Body temperatures were collected using a DAS-8007-P reader (BioMedic Data Systems) at a consistent daily time and a temperature of ≥39.5 °C was defined as fever74 (link)–76 (link).
8
Thermoregulation Effects of Uridine and Glucose
9
Body Composition and Metabolic Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Accurate determination of body composition was conducted using nuclear magnetic resonance (Bruckner Minispec mq10). Metabolic cage studies were performed in the UTSW Mouse Metabolic Phenotyping Core using the CLAMS system (Columbus Instrument) as described previously [11] (link). Prior to measurements, mice were allowed to acclimatize to the metabolic cages for five days. CT-Scanning was performed with a MicroCT Scanner (GE Healthcare) in the UTSW Mouse Metabolic Phenotyping Core. Body temperature was measured using an IPTT-300 transponder implanted longitudinally above the shoulder of mouse (Bio Medic Data Systems).
10
Transgenic Murine Model for SARS-CoV-2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult (10–13 week old) male and female K18-hACE2 transgenic mice (B6.Cg-Tg(K18-ACE2)2Prlmn/J) were obtained from Jackson Laboratories (Bar Harbor, ME). Mice were housed in groups of 4 in solid–bottom polysulfone individually ventilated cages (Allentown BCU) in rooms maintained on a 12:12-hour light:dark cycle at 22 ± 2°C with 30–70% humidity in the Regional Biocontainment Laboratory (animal biosafety level 3 facility) at UTHSC (Memphis, TN). Mice were acclimated for 1 day before being lightly anesthetized with 2% inhaled isoflurane (Baxter, Deerfield, IL) and implanted subcutaneously with an IPTT300 transponder (Bio Medic Data Systems, Seaford, DE) for identification and temperature monitoring, followed by an additional 3 days of acclimation before inclusion in the experiments. Envigo irradiated rodent diet (catalog no. 7912) and autoclaved water were available ad libitum during the acclimation and study periods; gel food and hydrogel were provided at the time of infection. All experimental procedures were performed under protocol 20–0132 approved by the Animal Care and Use Committee at University of Tennessee Health Science Center (UTHSC) under relevant institutional and American Veterinary Medical Association (AVMA) guidelines and were performed in a biosafety level 3 facility that is accredited by the American Association for Laboratory Animal Science (AALAS).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!