E el r0019

Manufactured by Elabscience

Sourced in China, United States

The E-EL-R0019 is a laboratory equipment product. It is designed to perform a specific core function, though the detailed description is not available at this time.

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Lab products found in correlation

E el r0015, by Elabscience (5 mentions) E el r0674, by Elabscience (2 mentions) E el r0098, by Elabscience (1 mentions) E el r0064, by Elabscience (1 mentions) E el r0506, by Elabscience (1 mentions) E bc k013 s, by Elabscience (1 mentions)

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E-EL-R0019/TNF-α (5 citations)

12 protocols using e el r0019

Serum biomarkers of myocardial injury

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Serum Troponin I and creatine kinase muscle-brain (CK-MB) analysis was made by Chemiluminescent micro particle immunoassay (CMIA) method with Autoanalyser (C8000 Architect, Abbott, Abbott Park, IL, U.S.A.) on the same day of sampling¹⁸ .
The serum concentrations of IL-6 and TNF-α were measured by performing enzyme-linked immunosorbent assay (ELISA) analyses. Inflammatory mediators from each sample were quantified using rat specific ELISA kits (E-EL-R0019, Elabscience Biotechnology Co. WuHan, PRC). Results were calculated using ELISA micro plate reader (DAR 800, Diagnostic Automation, CA 91302, USA) using standard curves and expressed as picograms per milliliter (pg/mL) of serum.

Durmaz S., Kurtoğlu T., Barbarus E., Eliyatkın N, & Yılmaz M. (2020). TNF-alpha inhibitor adalimumab attenuates endotoxin induced cardiac damage in rats. Acta Cirúrgica Brasileira, 35(2), e202000202.

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Rat TNF-α Quantification by ELISA

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TNF-α concentration was measured using a TNF-α ELISA kit for rats (E-EL-R0019; Elabscience). Briefly, 100 μl of the standard or sample was added to each well and incubated for 90 min. After the removal of the liquid, the wells were replenished with the same amount of biotinylated detection antibody. Thereafter, incubation was carried out for 1 h followed by aspiration and 3 rounds of washing. A 100 μl volume of the HRP conjugate was added to each well and incubated for 30 min. Thereafter, the wells were aspirated, washed 5 times, and treated with 90 μl of the substrate reagent. After incubation for 15 min, 50 μl of the stop solution was added, and absorbance was measured at 450 nm.

Lee H.Y., Nam Y., Choi W.S., Kim T.W., Lee J, & Sohn U.D. (2020). The hepato-protective effect of eupatilin on an alcoholic liver disease model of rats. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 24(5), 385-394.

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Quantitative ELISA Analysis of NF-κB, TNF-α, and IL-6

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The adipose levels of NF-κB, TNF- α and IL-6 were determined by quantitative standard sandwich ELISA technique using a monoclonal antibody specific for these parameters using rat kits with cat number E-EL-R0674, E-EL-R0019 and E-EL-R0015 respectively obtained from Elabscience Biotechnology Inc. (Wuhan, Hubei, P.R.C., China).

Areloegbe S.E., Peter M.U., Oyeleke M.B, & Olaniyi K.S. (2022). Low-dose spironolactone ameliorates adipose tissue inflammation and apoptosis in letrozole-induced PCOS rat model. BMC Endocrine Disorders, 22, 224.

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Colonic Cytokine Profiling by ELISA

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Colonic inflammatory cytokine concentrations of IL-13 (CAT# BEK1121, Chongqing Biospes Co., Ltd, China), and IL-1β (CAT# E-EL-R0019, Elabscience Biotechnology Inc., USA) were assessed using ELISA kits following the instructions provided by the manufacturer. Shortly, the procedure involved combining the colon tissue homogenates with purified mice IL-13 and IL-1β antibodies, which were then coated onto microliter plates. Following several washes, (TMB) substrate solution was added to produce a blue color following reaction with horseradish peroxidase. After 15 min, the stop solution was added and the produced yellow color was determined by utilizing a Robonik Readwell ELISA reader (India) at 450 nm.

Abdel-Razek E.A., Mahmoud H.M, & Azouz A.A. (2023). Management of ulcerative colitis by dichloroacetate: Impact on NFATC1/NLRP3/IL1B signaling based on bioinformatics analysis combined with in vivo experimental verification. Inflammopharmacology, 32(1), 667-682.

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Serum Biomarker Analysis in Rats

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After experiment completion, rats of each group were euthanized by cervical decapitation. Blood samples and paw tissues were collected. Blood was collected in a vacuum vessel containing no anticoagulant, centrifuged it at 3000 rpm for 4 min (min), and a serum sample was obtained. Further, serum samples were analyzed by an automatic hemocytometer to examine suppression of biochemical biomarkers C-reactive protein (CRP) and rheumatoid factor (RF). The ELISA (Enzyme-Linked Immunosorbent Assay) was also performed using a kit protocol (Elabscience, E-EL-R0019, and E-EL-R0015 catalog numbers) to calculate tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) concentration in blood serum [49 (link)].

Nazar N., Hussain A.I, & Rathore H.A. (2023). Inter-Varietal Variation in Phenolic Profile, Antioxidant, Anti-Inflammatory and Analgesic Activities of Two Brassica rapa Varieties: Influence on Pro-Inflammatory Mediators. Molecules, 29(1), 117.

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Quantifying Inflammatory Cytokine and Apoptosis Biomarkers

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The pro-inflammatory cytokine TNF-α (pg/ml) and pro-apoptosis protein Bax (ng/ml) were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits (Elabscience® USA catalogue E-EL-R0019 and E-EL-R0098 respectively) according to manufacturers’ protocol.

Suleman Z., Engwa G.A., Shauli M., Musarurwa H.T., Katuruza N.A, & Sewani-Rusike C.R. (2022). Neuroprotective effects of Lippia javanica (Burm.F.) Spreng. Herbal tea infusion on Lead-induced oxidative brain damage in Wistar rats. BMC Complementary Medicine and Therapies, 22, 4.

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Cytokine Quantification by ELISA

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The quantification of cytokines was performed by ELISA kit method following the kit instructions (Cat No: E-EL-R0674; E-EL-R0019; Elabscience, Texas, USA). Optical density was read at 415 nm in an ELISA reader (Erba, Germany, Amable et al., 2013 , Kim et al., 2016 (link)).

Ittiyavirah S.P., Ramalingam K., Sathyan A., Rajasree R.S., Kuruniyan M.S., Quadri S.A., Elayadeth-Meethal M, & Naseef P.P. (2022). Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar rats. Saudi Pharmaceutical Journal : SPJ, 30(12), 1781-1790.

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Measuring Rat TNF-α Levels

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The effects of the extracts on TNF-α levels after 48 h treatment were determined on the culture media using the rat tumor necrosis factor alpha (TNF-α) ELISA kit (E-EL-R0019, Elabscience, Biocom Africa) according to the manufacture manual protocol. Concentrations of rat TNF-α concentration in the cell culture media samples were calculated from the standard curve.

Nkadimeng S.M., Steinmann C.M, & Eloff J.N. (2020). Effects and safety of Psilocybe cubensis and Panaeolus cyanescens magic mushroom extracts on endothelin-1-induced hypertrophy and cell injury in cardiomyocytes. Scientific Reports, 10, 22314.

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Quantifying Inflammatory Cytokines in Serum

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ELISA was performed to determine the concentrations of tumor necrosis factor and interleukin 6 in serum using kit protocols (Elabscience, Catalog Number E-EL-R0019 and E-EL-R0015) (Cheng et al., 2015 (link)).

Manan M., Saleem U., Ahmad B., Aslam N., Anwar A, & Zafar A. (2022). Anti-arthritic and toxicological evaluation of ethanolic extract of Alternanthera bettzickiana in rats. Frontiers in Pharmacology, 13, 1002037.

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Quantification of Pro-Inflammatory Cytokines

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The supernatant cells were used to determine the levels of pro-inflammatory cytokine expression in the L2 cells, such as TNF-α, CRP, and IL-12. The TNF-α, CRP, and IL-12 levels were determined by the ELISA kit (Elabscience E-EL-R0019, E-EL-R0506, and E-ELR0064, respectively). The process was carried out according to the manufacturer’s guidelines. The sample absorbances were tested at a wavelength of 450 nm⁸.

Priyandoko D., Widowati W., Lenny L., Novianti S., Revika R., Kusuma H.S, & Sholihah I.A. (2024). Green Tea Extract Reduced Lipopolysaccharide-Induced Inflammation in L2 Cells as Acute Respiratory Distress Syndrome Model Through Genes and Cytokine Pro-Inflammatory. Avicenna Journal of Medical Biotechnology, 16(1), 57-65.

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