Cd39 pe

Manufactured by BioLegend

Sourced in United States

CD39-PE is a fluorescently labeled antibody that binds to the CD39 (Ecto-NTPDase1) cell surface antigen. CD39 is an ectonucleotidase that hydrolyzes extracellular nucleotides. This product can be used for flow cytometric analysis of cells expressing CD39.

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Anti-CD39-PE (2 citations)

3 protocols using cd39 pe

Alginate Hydrogel Preparation and Characterization

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Alginate (CAS: 9005-38-3) was purchased from Meilunbio (Dalian, China). All anti-mouse antibodies including CD86-PE (#105008), CD11c-APC (#117310), I-A/I-E-PercP Cyanine 5.5 (#107626), CD73-APC (#127210), CD39-PE (#143804), Ly-6A/E (Sca-1)-FITC (#108105), CD44-PE (#103007), CD45-FITC (#103108), PDL1-PE Cy7 (#124314), and CD11b-FITC (#101206) were procured from Biolegend (San Diego, CA, USA). Calcein AM and propidium iodide (PI) were purchased from Immunochemistry (Lumington, CA, USA). Recombinant murine IL-4 (#214-14) and GM-CSF (#315-03) were obtained from PeproTech (Rocky Hill, NJ, USA), LPS-EB (CAS: 5969-42-02), and lipopolysaccharide from Escherichia coli 0111: B4 strain was purchased from Invivo Gen (Hong Kong, China). The selective adenosine A_2A receptor (A_2AR) antagonist SCH58261 (HY-19533) and the selective adenosine A_2B receptor (A_2BR) antagonist LAS01057 (HY-14390) were purchased from MedChemExpress (Shanghai, China). The DiR cell membrane fluorescent probe (MB12482, Meilunbio) was procured from Meilunbio. The Toxin Sensor™ Chromogenic LAL Endotoxin Assay Kit (GenScript, Piscataway, NJ, USA) was used to exclude endotoxin contamination during hydrogel preparation. The endotoxin level in the Alginate hydrogel was lower than 0.1 endotoxin unit/mL.

Shi G., Zhou Y., Liu W., Chen C., Wei Y., Yan X., Wu L., Wang W., Sun L, & Zhang T. (2023). Bone-derived MSCs encapsulated in alginate hydrogel prevent collagen-induced arthritis in mice through the activation of adenosine A2A/2B receptors in tolerogenic dendritic cells. Acta Pharmaceutica Sinica. B, 13(6), 2778-2794.

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Flow Cytometry Analysis of T Cell Phenotype

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For surface staining, in vitro cultured human T cells or NCG mice-retrieved PBMCs were stained for 30 min at 4 °C with the following antibodies at 1:200 dilution: CD8a PE/Cyanine7 (BioLegend, 344711), PD-1 PB (BioLegend, 329915), TIM-3 FITC (BioLegend, 345021), and CD39 PE (BioLegend, 328207). Intracellular staining was performed as described above and the flowing antibodies were used at 1:200 dilution: TNFα APC (BioLegend, 502913), IFNγ FITC (BioLegend, 502505), IL-2 PE (BioLegend, 500306), Ki-67 PerCP/Cyanine5.5 (BD Pharmingen, 561284), and Granzyme-B AF647 (BioLegend, 515405). Samples were run on a BD LSRFortessa and data were analyzed using FlowJo (10.4).

Qiu F., Jiang P., Zhang G., An J., Ruan K., Lyu X., Zhou J, & Sheng W. (2024). Priming with LSD1 inhibitors promotes the persistence and antitumor effect of adoptively transferred T cells. Nature Communications, 15, 4327.

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Phenotypic Analysis of T Cell Subsets

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For phenotypic analysis of T cells, PBMCs were stained with CD3-PE, CD4-APC, CD45RA−FITC, and CD28-PerCP/Cy5 antibodies or isotype controls (BioLegend, San Diego, CA). CD39-PE and CD57-APC (BioLegend) were employed to assess senescent status of CD4 T cells. To determine cell apoptosis, PBMCs were stained with CD45RA−FITC, CD4-APC along with Annexin V (Av)-PE and 7-aminoactinomycin D (7AAD) (BD Biosciences, San Jose, CA) following the manufacturer’s protocol. Reactive oxygen species (ROS) were measured using the 2ʹ,7ʹ-Dichlorofluorescin Diacetate (DCFDA)−based Cellular ROS Detection Kit (Abcam, Cambridge, MA) according to manufacturer’s protocol. Flow cytometric analysis, gating strategy, and background controls were performed as described previously^{6 (link)}.

Nguyen L.N., Zhao J., Cao D., Dang X., Wang L., Lian J., Zhang Y., Jia Z., Wu X.Y., Morrison Z., Xie Q., Ji Y., Zhang Z., El Gazzar M., Ning S., Moorman J.P, & Yao Z.Q. (2018). Inhibition of TRF2 accelerates telomere attrition and DNA damage in naïve CD4 T cells during HCV infection. Cell Death & Disease, 9(9), 900.

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