The absorption spectra of the extracts were evaluated with wavelengths in the range 200–700 nm by means of an ultraviolet (UV)-visible spectrophotometer (SPECTROstar Nano; BMG LABTECH, Offenburg, Germany) (22 (link), 23 (link)). For, 1H nuclear magnetic resonance (NMR) spectra, the extracts (1 mg) were dissolved in deuterated dimethyl sulfoxide (DMSO-d6)/deuterium oxide (D2O) and the spectral data were recorded on an Avance 300 MHz NMR spectrometer (Bruker, Rheinstetten, Germany). Structural elucidation of the pigments was accomplished by comparison of the 1H NMR spectra as described by Choi et al. (22 (link)) and Sobolev et al. (24 (link)).
Avance 300 mhz nmr spectrometer
Manufactured by Bruker
Sourced in United States
The Avance 300 MHz NMR spectrometer is a laboratory instrument designed for nuclear magnetic resonance (NMR) analysis. It operates at a frequency of 300 MHz and is capable of performing various NMR spectroscopy techniques to study the structure and properties of chemical compounds.
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Lab products found in correlation
Agilent 1100 lc msd esi mass spectrum, by Agilent Technologies (2 mentions)
Spectrostar nano, by BMG Labtech (1 mentions)
Nicolet is10 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions)
Sdt 650, by TA Instruments (1 mentions)
Avance 600 mhz nmr, by Bruker (1 mentions)
Phenom prox sem, by Thermo Fisher Scientific (1 mentions)
Solvents and reagents, by Merck Group (1 mentions)
100 ftir spectrometer, by PerkinElmer (1 mentions)
Ftir spectrometer, by PerkinElmer (1 mentions)
8453 uv vis spectrophotometer, by Agilent Technologies (1 mentions)
Chns 932 elemental analyzer, by Leco (1 mentions)
Nicolet 6700 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions)
Waters 2695 separations module, by Waters Corporation (1 mentions)
Heleos 2 multi angle laser light scattering detector, by Wyatt Technology (1 mentions)
Optilab t rex refractive index detector, by Wyatt Technology (1 mentions)
Model 1000, by PerkinElmer (1 mentions)
Dsc 822, by Mettler Toledo (1 mentions)
14 protocols using avance 300 mhz nmr spectrometer
1
Spectroscopic Analysis of Natural Pigments
2
Comprehensive Material Characterization Protocol
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A ThermoFisher Scientific (Waltham, MA, USA) Nicolet iS 10 FTIR spectrometer was used for FTIR characterizations. 1D 1H and 13C NMR were performed using a Bruker (Billerica, MA, USA) Avance 300 MHz NMR spectrometer, while 2D 1H-1H COSY and 1H-13C HMBC NMR were performed on a Bruker (Billerica, MA, USA) Avance 600 MHz NMR. All NMR spectra were collected in D2O. Thermogravimetric analysis was performed using a TA Instruments (New Castle, DE, USA) simultaneous thermal analyser (SDT 650) under nitrogen atmosphere. Data were collected from 30 to 800°C at a heating rate of 10°C min−1 after water was removed through equilibration at 110°C for 4 min. Scanning electron microscopy (SEM) images were obtained in backscatter mode using a Phenom ProX SEM (Phenom-World B.V., Eindhoven, Netherlands).
3
NMR Spectroscopy Protocol for Solvent Samples
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1H NMR experiments were performed on a Bruker Avance 300 MHz NMR Spectrometer (Bruker, Billerica, MA, USA). The samples were prepared in D2O, chloroform-d, or DMSO-d6 solution at 20 mg/mL and the experiment relaxation delay (d1) was 2 seconds.
4
Radiolabeled RGD Peptide Synthesis
5
Synthesis and Characterization of Novel Compounds
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All the reagents were purchased from commercial suppliers and were used without further purification unless otherwise indicated. All the reactions were monitored by thin-layer chromatography (TLC) on silica gel precoated F254 Merck plates, and spots were examined under UV light (254 nm). All column chromatography was performed using 200-300 mesh silica gel. 1H NMR and13C NMR spectra were taken on a Bruker Avance 300-MHz NMR Spectrometer at 300 K with TMS as the internal standard, and CDCl3 and DMSO-d6 were used as solvent, the values of the chemical shifts (δ) are expressed in parts per million (ppm), and coupling constants (J) are expressed in hertz (Hz). MS spectra were recorded on an Agilent 1100 LC/MSD (ESI) Mass Spectrum.
6
Characterization of Compound 5b by NMR and IR
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1H NMR and 13C NMR spectra were recorded on Bruker Avance 300 MHz NMR Spectrometer in either CDCl3 or DMSO-d6 or CDCl3 with few drops of DMSO-d6 at 298 K. Chemical shifts (δ) were reported in parts per million (ppm) with respect to internal standard TMS and coupling constants (J) are denoted in Hz. Proton multiplicity is assigned using the following abbreviations: singlet (s), doublet (d), triplet (t), quartet (q), multiplet (m). FT-IR spectra of the compound 5b in powder and xerogel form were recorded using Perkin Elmer 100 FTIR Spectrometer in the spectral range of 4000 to 500 cm−1.
7
NMR Characterization of Molecules
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1H NMR and 13C NMR experiments were carried out by a Bruker Avance 300 MHz NMR spectrometer in DMSO-d6 using tetramethyl silane (TMS) as internal standard, and the chemical shift values of hydrogen atoms and carbon atoms were reported in parts per million (ppm), respectively.
8
Comprehensive Characterization of Synthesized Compounds
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The Agilent 8453 UV–vis
spectrophotometer was utilized to record the UV–visible spectra.
The temperature of the cell was kept fixed by an automated thermostat.
The Bruker Avance 300 MHz NMR spectrometer was used for 1H-NMR measurements with D2O as a solvent at 298.15 K.
HRMS spectra were recorded with positive-mode electrospray ionization
in high-resolution Q-TOF instrument.
The Perkin-Elmer FT-IR
spectrometer was used to measure the IR
spectra using the KBr disk method at room temperature.
The Bruker
D8 advance powder X-ray diffractometer was used to record
powder XRD experiments. A JSM-6360 scanning electron microscope (SEM)
was used for the surface morphological analysis.
spectrophotometer was utilized to record the UV–visible spectra.
The temperature of the cell was kept fixed by an automated thermostat.
The Bruker Avance 300 MHz NMR spectrometer was used for 1H-NMR measurements with D2O as a solvent at 298.15 K.
HRMS spectra were recorded with positive-mode electrospray ionization
in high-resolution Q-TOF instrument.
The Perkin-Elmer FT-IR
spectrometer was used to measure the IR
spectra using the KBr disk method at room temperature.
The Bruker
D8 advance powder X-ray diffractometer was used to record
powder XRD experiments. A JSM-6360 scanning electron microscope (SEM)
was used for the surface morphological analysis.
9
Characterizing Oxoiron(IV) Complexes via 2H NMR
10
Synthesis and Characterization of Organometallic Complexes
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Ferrocene, hydrochloric acid, sodium nitrite, acetonitrile, dimethyl sulfoxide (DMSO), ethanol, diethyl ether, carbon disulfide, triethylamine, metal salts (Pd, Ag, Cd, Zn, Hg, etc.), ammonium formate, zinc-dust, and alkaline phosphatase (ALP, EC 3.1.3.1) were obtained from E. Merck and Aldrich (Pakistan). All solvents were dried and purified before use according to the reported methods [18 (link)]. Elemental analyses (CHNS) were performed using an in-house instrument, Leco CHNS-932 Elemental Analyzer. Melting points were measured using a BIO COTE Model SMP10 melting point apparatus. The FT-IR spectra (4,000–400) cm−1 were obtained using KBr disks on a Thermo Scientific Nicolet-6700 FT-IR spectrometer. The NMR spectra of the complexes were recorded using a Bruker Avance 300 MHz NMR spectrometer.
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