Cells were prepared for apoptosis analysis by resuspending cell pellets in 100 μl 1× Annexin binding buffer as provided by the Tali™ Apoptosis Kit- Annexin V Alexa Fluor™ 488 & Propidium Iodide (Life Technologies). Five μl of Annexin V Alexa Fluor® 488 were added to each sample before incubation in the dark for 20 min. Cells were spun down at 500 rcf for 10 min before resuspending in 100 μl 1× Annexin binding buffer. One μl of Tali® Propidium Iodide was added to each tube before incubating in the dark for 5 min. Samples were taken to the UAB Flow Cytometry Core for analysis.
Tali apoptosis kit annexin 5 alexa fluor 488 propidium iodide
Manufactured by Thermo Fisher Scientific
Sourced in Spain
The Tali™ Apoptosis Kit - Annexin V Alexa Fluor™ 488 & Propidium Iodide is a fluorescence-based assay that enables the detection and quantification of apoptotic cells. The kit contains Annexin V conjugated to the Alexa Fluor™ 488 fluorophore and propidium iodide, which are used to identify early and late apoptotic cells, respectively.
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Lab products found in correlation
2 protocols using tali apoptosis kit annexin 5 alexa fluor 488 propidium iodide
1
Annexin V and PI Apoptosis Assay
2
Apoptosis Assay with Annexin-V and PI
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Annexin-V/propidium iodide (PI) double staining assay was performed using the Tali® Apoptosis Kit—Annexin V Alexa Fluor® 488 & Propidium Iodide according to the manufacturer's instructions (Life Technologies, Madrid, Spain). Briefly, after being treated with IC50 and IC90 of the tested molecules for 24 h, amoebae were centrifuged at 250 g for 10 minutes, washed twice with the Annexin Binding Buffer (ABB) and incubated with 5 μl of annexin-V for 20 min. After that, cells were centrifuged and resuspended in ABB containing 1 μl of PI and incubated for 3 min at room temperature. Finally, 25 μl of the stained cells were loaded into a Tali® Cellular Analysis Slide and were analysed in the Tali® Image-Based Cytometer. Data were collected using the Tali® data acquisition and analysis software (Life Technologies, Madrid, Spain) [17 (link)].
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