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Ritanserin

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Ritanserin is a selective serotonin 2A (5-HT2A) and serotonin 2C (5-HT2C) receptor antagonist. It is used as a pharmacological tool to study the role of 5-HT2A and 5-HT2C receptors in various biological processes.

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Lab products found in correlation

Ketanserin tartrate, by Bio-Techne (2 mentions) Arachidonic acid d8, by Cayman Chemical (2 mentions) 1 stearoyl 2 arachidonoyl d8 sn glycerol sag d8, by Cayman Chemical (2 mentions) Aa d8, by Cayman Chemical (2 mentions) Polyethyleneimine, by Polysciences (2 mentions) Capsaicin, by Merck Group (1 mentions) Chloroquine, by Merck Group (1 mentions) 5 n ethyl n isopropyl amiloride eipa, by Merck Group (1 mentions) Ps6 ribosomal, by Cell Signaling Technology (1 mentions) S6 ribosomal protein, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Rapamycin, by LC Laboratories (1 mentions) Actin, by Merck Group (1 mentions) Phosphatidic acid, by Avanti Polar Lipids (1 mentions) Torin1, by LC Laboratories (1 mentions) Sb204741, by Bio-Techne (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions)

7 protocols using ritanserin

1

Serotonin and Capsaicin Receptor Antagonism

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5-HT and capsaicin were purchased from Sigma-Aldrich (St. Louis, MI, USA). The 5-HT2 receptor antagonist, ritanserin, and the 5-HT3 receptor antagonist, Y-25130 hydrochloride, were purchased from Tocris Bioscience. Concentrations of chemicals used in this study were based on reported IC50 of antagonists and previous studies.39 (link) All drugs except for capsaicin were dissolved in water to prepare 1 or 10 mm stock solutions. capsaicin was diluted in DMSO to 10 mm stock solution. All drugs underwent further dilutions in the bath solution. Final DMSO concentration was < 0.01%.
Kullmann F.A., Chang H.H., Gauthier C., McDonnell B.M., Yeh J.C., Clayton D.R., Kanai A.J., de Groat W.C., Apodaca G.L, & Birder L.A. (2017). Serotonergic paraneurones in the female mouse urethral epithelium and their potential role in peripheral sensory information processing. Acta physiologica (Oxford, England), 222(2), 10.1111/apha.12919.
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2

Lipid Signaling Pathway Reagents

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Unless otherwise specified, all reagents were purchased from Fisher Scientific. Polyethyleneimine (Polysciences Inc., Cat# 24765), ritanserin ≥99% by HPLC (Tocris Bioscience, Cat# 1955), ketanserin tartrate ≥97% by HPLC (Tocris Bioscience, Cat# 0908), 1-stearoyl-2-arachidonoyl-d8-sn-glycerol (SAG-d8; Cayman Chemical Company, Cat# 10009872), and arachidonic acid-d8 (AA-d8; Cayman Chemical Company, Cat# 390010).
Ware T.B., Franks C.E., Granade M.E., Zhang M., Kim K.B., Park K.S., Gahlmann A., Harris T.E, & Hsu K.L. (2020). Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering. Nature chemical biology, 16(2), 170-178.
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3

Ritanserin Pretreatment for JCPyV Infection

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Treatment with ritanserin was performed as described previously (O’Hara and Atwood, 2008 (link)). Briefly, cells at 60% confluence were pretreated with ritanserin (Tocris Bioscience) at 25 µM concentration O/N in complete medium at 37°c. The cells were then prechilled for 30 mins and infected on ice with JCPyV for 1 h in plain MEM 1X. The cells were washed with 1X PBS and warmed MEM with 10% FBS, antibiotics and ritanserin at 25 µM was added. The cells were stained for VP1 after 72 hr as described above.
Assetta B., Morris-Love J., Gee G.V., Atkinson A.L., O’Hara B.A., Maginnis M.S., Haley S.A, & Atwood W.J. (2019). Genetic and Functional Dissection of the Role of Individual 5-HT2 Receptors as Entry Receptors for JC Polyomavirus. Cell reports, 27(7), 1960-1966.e6.
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4

Protein Expression and Autophagy Regulation

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The following antibodies were used: Tsc2, pS6 ribosomal, S6 ribosomal protein, PARP, Cleaved Caspase 3, (Cell Signaling Technology), actin (Sigma-Aldrich). Rapamycin and Torin1 were purchased from LC Laboratories. Chloroquine and 5-(N-Ethyl-N-isopropyl) amiloride (EIPA) were purchased from Sigma-Aldrich. Ritanserin was purchased from Tocris (Cat. No. 1955). Phosphatidic acid was purchased from Avanti Polar Lipids.
Kovalenko A., Sanin A., Kosmas K., Zhang L., Wang J., Akl E.W., Giannikou K., Probst C.K., Hougard T.R., Rue R.W., Krymskaya V.P., Asara J.M., Lam H.C., Kwiatkowski D.J., Henske E.P, & Filippakis H. (2021). Therapeutic targeting of DGKA-mediated macropinocytosis leads to phospholipid reprogramming in Tuberous Sclerosis Complex. Cancer research, 81(8), 2086-2100.
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5

Ritanserin Pretreatment for JCPyV Infection

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Treatment with ritanserin was performed as described previously (O’Hara and Atwood, 2008 (link)). Briefly, cells at 60% confluence were pretreated with ritanserin (Tocris Bioscience) at 25 µM concentration O/N in complete medium at 37°c. The cells were then prechilled for 30 mins and infected on ice with JCPyV for 1 h in plain MEM 1X. The cells were washed with 1X PBS and warmed MEM with 10% FBS, antibiotics and ritanserin at 25 µM was added. The cells were stained for VP1 after 72 hr as described above.
Assetta B., Morris-Love J., Gee G.V., Atkinson A.L., O’Hara B.A., Maginnis M.S., Haley S.A, & Atwood W.J. (2019). Genetic and Functional Dissection of the Role of Individual 5-HT2 Receptors as Entry Receptors for JC Polyomavirus. Cell reports, 27(7), 1960-1966.e6.
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6

Chiral Praziquantel Protocol

Cited 1 time
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Ritanserin, SB204741 and mesulergine were from Tocris Bioscience. All other ligands were from Sigma-Aldrich. Cell culture reagents were from Invitrogen. PZQ enantiomers ((R)-[-]PZQ and (S)-[+]PZQ) were resolved (Supplementary Fig. 2) using methods published by Woelfle et al.13 (link).
Chan J.D., Cupit P.M., Gunaratne G.S., McCorvy J.D., Yang Y., Stoltz K., Webb T.R., Dosa P.I., Roth B.L., Abagyan R., Cunningham C, & Marchant J.S. (2017). The anthelmintic praziquantel is a human serotoninergic G-protein-coupled receptor ligand. Nature Communications, 8, 1910.
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7

Lipid Signaling Pathway Reagents

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Unless otherwise specified, all reagents were purchased from Fisher Scientific. Polyethyleneimine (Polysciences Inc., Cat# 24765), ritanserin ≥99% by HPLC (Tocris Bioscience, Cat# 1955), ketanserin tartrate ≥97% by HPLC (Tocris Bioscience, Cat# 0908), 1-stearoyl-2-arachidonoyl-d8-sn-glycerol (SAG-d8; Cayman Chemical Company, Cat# 10009872), and arachidonic acid-d8 (AA-d8; Cayman Chemical Company, Cat# 390010).
Ware T.B., Franks C.E., Granade M.E., Zhang M., Kim K.B., Park K.S., Gahlmann A., Harris T.E, & Hsu K.L. (2020). Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering. Nature chemical biology, 16(2), 170-178.
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