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Lsm700 800

Manufactured by Leica
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The LSM700/800 is a confocal laser scanning microscope system designed for high-resolution imaging. It provides precise control over the illumination and detection of samples, enabling detailed analysis of microscopic structures.

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Lab products found in correlation

Lsm 780, by Zeiss (4 mentions) Sp5ii, by Leica (2 mentions) Propidium iodide, by Merck Group (2 mentions)

Close spelling variants of this product

LSM700

2 protocols using lsm700 800

1

Imaging of plant root development

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Roots were stained by propidium iodide (PI; Sigma-Aldrich) at final concentration of 10μg/ml. Embryos were fixed and stained by 2.2% Renaissance RS2200 (Renaissance Chemicals Ltd) in PBS buffer at pH 6.9 containing 4% paraformaldehyde, 5% glycerol, 4.2% dimethyl sulfoxide (DMSO) or stained by PI after fixation as previously described6 (link),38 (link).
Confocal imaging was performed with Zeiss LSM700/800 (observation of lateral root) or Leica SP5/II (observation of primary root and embryo) as previously described38 (link). Live imaging was performed with LSM700 as described before39 (link),40 (link). For the 3D imaging of pSOK1-SOK1-YFP and pSOK1-GFP root, Zeiss LSM780 with 2 photon laser (960-990nm) and 500-550nm/575-610nm band-pass filter were used. Analysis of confocal images (3D reconstruction, 3D segmentation) was performed by MorphoGraphX 41 (link).
Yoshida S., van der Schuren A., van Dop M., van Galen L., Saiga S., Adibi M., Möller B., ten Hove C.A., Marhavy P., Smith R., Friml J, & Weijers D. (2019). A SOSEKI-based coordinate system interprets global polarity cues in Arabidopsis. Nature plants, 5(2), 160-166.
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2

Imaging of plant root development

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Roots were stained by propidium iodide (PI; Sigma-Aldrich) at final concentration of 10μg/ml. Embryos were fixed and stained by 2.2% Renaissance RS2200 (Renaissance Chemicals Ltd) in PBS buffer at pH 6.9 containing 4% paraformaldehyde, 5% glycerol, 4.2% dimethyl sulfoxide (DMSO) or stained by PI after fixation as previously described6 (link),38 (link).
Confocal imaging was performed with Zeiss LSM700/800 (observation of lateral root) or Leica SP5/II (observation of primary root and embryo) as previously described38 (link). Live imaging was performed with LSM700 as described before39 (link),40 (link). For the 3D imaging of pSOK1-SOK1-YFP and pSOK1-GFP root, Zeiss LSM780 with 2 photon laser (960-990nm) and 500-550nm/575-610nm band-pass filter were used. Analysis of confocal images (3D reconstruction, 3D segmentation) was performed by MorphoGraphX 41 (link).
Yoshida S., van der Schuren A., van Dop M., van Galen L., Saiga S., Adibi M., Möller B., ten Hove C.A., Marhavy P., Smith R., Friml J, & Weijers D. (2019). A SOSEKI-based coordinate system interprets global polarity cues in Arabidopsis. Nature plants, 5(2), 160-166.
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