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Anti trek 1

Manufactured by Alomone
Sourced in Israel

Anti-TREK-1 is a laboratory tool used to detect and quantify the expression of the TREK-1 (KCNK2) protein. It is a specific antibody designed for use in various experimental techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to analyze the presence and levels of TREK-1 in biological samples.

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2 protocols using anti trek 1

1

Biochemical and Electrophysiological Analysis of TREK-1 and BDNF Signaling

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The ELISA kit to measure corticosterone was from Enzo Life Science. Rabbit polyclonal antibodies anti-TREK-1 and anti-proBDNF were from Alomone Labs (Israel). Rabbit polyclonal antibodies anti-furin and anti-NaKATPase, the mouse monoclonal anti-beta-actin and goat anti-TGN38 and anti-DCX antibodies were from SantaCruz Technologies (United States). Rabbit polyclonal antibodies anti-TrkB and anti-phospho-(Tyr 705)-TrkB were from Signalway Antibody (United States). Rabbit polyclonal antibodies against BDNF and plasminogen were from GeneTex (United States). Mouse monoclonal anti-BrdU antibody was from Becton Dickinson (United States). Anti-rabbit, anti-mouse, and anti-goat secondary antibodies were from Cell Signaling Technologies. HRP substrate (Immobilon Forte, Millipore), Nitrocellulose (BioTrace NT, Life Sciences), SDS-PAGE gels and Mini Protean apparatus were from Biorad. BrdU was from Interchim (France). Neurobasal medium, B-27 and Penicillin-Streptomycin were from Thermo Fisher Scientific. Poly-D-Lysine was from Sigma-Aldrich. Patch clamp was performed on a pClamp 8.2 apparatus (Molecular Devices)
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2

Immunohistochemistry of TREK-1 in Mouse Brain

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Mouse brain were obtained following perfusion with 4% PFA in PBS solution, and 40 µm-thick free-floating sections were prepared using a vibratome. Sections were permeabilized with 0.5% Triton X-100 in PBS for 20 min at RT, followed by blocking with 5% donkey serum and 2% BSA with 0.3% Triton X-100 in PBS for 1 h at RT. Tissues were incubated overnight at 4 °C with primary antibodies. The following antibodies were used in this study: Anti-TREK-1 (Alomone labs, APC-047, Jerusalem, Israel). For detection, suitable Alexa fluor-tagged secondary antibody (Jackson ImmunoResearch) was used, and the tissues were counterstained with DAPI.
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