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Plan apo oil objective lens

Manufactured by Nikon
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The Plan Apo oil objective lens is a high-performance optical lens designed for use in microscopy applications. It provides a flat, distortion-free image with excellent resolution and contrast. The lens is optimized for use with immersion oil, which helps to improve image quality and minimize spherical aberrations.

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Lab products found in correlation

Coolsnap hq2 ccd camera, by Nikon (2 mentions)

Close spelling variants of this product

Plan Apo VC 60x 1.4 NA oil immersion objective lens Plan-Apo ×63/1.4 numerical aperture oil lens objective Plan Apo objective lens Plan Apo lens Plan Apo objective 10× objective lens Plan Apo

2 protocols using plan apo oil objective lens

1

Immunofluorescence Analysis of VSVG Trafficking

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence analysis, cells were fixed using paraformaldehyde (4%) and stained using primary antibodies at a final concentration of 1 μg/mL, as described previously (Johnson et al., 2015 (link)). Imaging was conducted on a swept-field confocal microscope using a Roper CoolSnap HQ2 CCD camera and a Nikon 603, 1.4 numerical aperture (NA) Plan Apo oil objective lens. Acquisition parameters were controlled by Nikon Elements, and image analysis was conducted using ImageJ or Imaris (Bitplane) software. BFA treatments (10 mg/mL) were each conducted for 1 hr at 37o C, followed by washout using pre-warmed media. Cells expressing GFP-tagged VSVG were incubated overnight at 40o C and subsequently placed into a Tokai Hit stage top incubator set to 32o C for live-cell imaging. Particle tracking, volume measurements, and linescan analysis for intensity measurements were conducted in an unbiased manner using Imaris software.
Slosarek E.L., Schuh A.L., Pustova I., Johnson A., Bird J., Johnson M., Frankel E.B., Bhattacharya N., Hanna M.G., Burke J.E., Ruhl D.A., Quinney K., Block S., Peotter J.L., Chapman E.R., Sheets M.D., Butcher S.E., Stagg S.M, & Audhya A. (2018). Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation. Cell reports, 24(9), 2248-2260.
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2

Immunofluorescence Analysis of VSVG Trafficking

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence analysis, cells were fixed using paraformaldehyde (4%) and stained using primary antibodies at a final concentration of 1 μg/mL, as described previously (Johnson et al., 2015 (link)). Imaging was conducted on a swept-field confocal microscope using a Roper CoolSnap HQ2 CCD camera and a Nikon 603, 1.4 numerical aperture (NA) Plan Apo oil objective lens. Acquisition parameters were controlled by Nikon Elements, and image analysis was conducted using ImageJ or Imaris (Bitplane) software. BFA treatments (10 mg/mL) were each conducted for 1 hr at 37o C, followed by washout using pre-warmed media. Cells expressing GFP-tagged VSVG were incubated overnight at 40o C and subsequently placed into a Tokai Hit stage top incubator set to 32o C for live-cell imaging. Particle tracking, volume measurements, and linescan analysis for intensity measurements were conducted in an unbiased manner using Imaris software.
Slosarek E.L., Schuh A.L., Pustova I., Johnson A., Bird J., Johnson M., Frankel E.B., Bhattacharya N., Hanna M.G., Burke J.E., Ruhl D.A., Quinney K., Block S., Peotter J.L., Chapman E.R., Sheets M.D., Butcher S.E., Stagg S.M, & Audhya A. (2018). Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation. Cell reports, 24(9), 2248-2260.
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