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Rat anti mouse cd40 1c10

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The Rat anti-mouse CD40 (1C10) is a primary antibody used in immunological research. It recognizes the mouse CD40 cell surface receptor, which plays a critical role in B-cell activation and regulation of the immune response. This antibody can be used for applications such as flow cytometry, immunoprecipitation, and immunohistochemistry to identify and study cells expressing the mouse CD40 antigen.

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Lab products found in correlation

Mem non essential amino acid, by Corning (2 mentions) Anti biotin microbead, by Miltenyi Biotec (2 mentions) Cd43 microbead, by Miltenyi Biotec (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Corning (2 mentions) Mouse ril 4, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igm f ab 2, by Jackson ImmunoResearch (2 mentions) Cd43 microbeads, by Thermo Fisher Scientific (2 mentions) Cd38 90 biotin, by Thermo Fisher Scientific (2 mentions) Cd11c n418 biotin, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igg f ab 2, by Jackson ImmunoResearch (2 mentions) Goat anti mouse κ f ab 2, by Southern Biotech (2 mentions) Mouse rbaff, by R&D Systems (2 mentions)

Spelling variants of this product

Anti-CD40 (70 citations) Anti-CD40 (1C10) (5 citations) CD40 (1C10, (2 citations)

2 protocols using rat anti mouse cd40 1c10

1

Isolation and Culture of Germinal Center B Cells

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B splenocytes were MACS-purified, collecting the negative fraction of CD43 Microbeads (Miltenyi Biotec). GC B cells were MACS-purified, collecting the negative fraction of CD43 Microbeads, CD38 (90) Biotin (Thermo Fisher Scientific), CD11c (N418) Biotin (Thermo Fisher Scientific) and anti-Biotin Microbeads (Miltenyi Biotec). Cells were cultured in RPMI 1640 (Corning) supplemented with 10% FBS (MilliporeSigma and Thermo Fisher Scientific), 1x Penicillin-Streptomycin, 1x MEM Nonessential Amino Acids (Corning), 1mM Sodium Pyruvate, 2mM GlutaMax, and 55 μM 2-Mercaptoethanol (Thermo Fisher Scientific). Cells were stimulated with the following reagents: 10μg/ml goat anti-mouse IgM F(ab’)2, 10μg/ml goat anti-mouse IgG F(ab’)2 (Jackson ImmunoResearch Labs), 10μg/ml goat anti-mouse κ F(ab’)2 (SouthernBiotech), 10ng/ml mouse rBaff (R&D Systems), 5μg/ml rat anti-mouse CD40 (1C10) and 10ng/ml mouse rIL-4 (Thermo Fisher Scientific). For in vitro-derived GC B cell cultures, B splenocytes were cultured on 40LB cells (3T3 fibroblasts expressing CD40L and secreting BAFF) with addition of 1ng/ml mouse rIL-4.
Ramezani-Rad P., Chen C., Zhu Z, & Rickert R.C. (2020). Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells. Cell reports, 33(7), 108403.
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2

Isolation and Culture of Germinal Center B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
B splenocytes were MACS-purified, collecting the negative fraction of CD43 Microbeads (Miltenyi Biotec). GC B cells were MACS-purified, collecting the negative fraction of CD43 Microbeads, CD38 (90) Biotin (Thermo Fisher Scientific), CD11c (N418) Biotin (Thermo Fisher Scientific) and anti-Biotin Microbeads (Miltenyi Biotec). Cells were cultured in RPMI 1640 (Corning) supplemented with 10% FBS (MilliporeSigma and Thermo Fisher Scientific), 1x Penicillin-Streptomycin, 1x MEM Nonessential Amino Acids (Corning), 1mM Sodium Pyruvate, 2mM GlutaMax, and 55 μM 2-Mercaptoethanol (Thermo Fisher Scientific). Cells were stimulated with the following reagents: 10μg/ml goat anti-mouse IgM F(ab’)2, 10μg/ml goat anti-mouse IgG F(ab’)2 (Jackson ImmunoResearch Labs), 10μg/ml goat anti-mouse κ F(ab’)2 (SouthernBiotech), 10ng/ml mouse rBaff (R&D Systems), 5μg/ml rat anti-mouse CD40 (1C10) and 10ng/ml mouse rIL-4 (Thermo Fisher Scientific). For in vitro-derived GC B cell cultures, B splenocytes were cultured on 40LB cells (3T3 fibroblasts expressing CD40L and secreting BAFF) with addition of 1ng/ml mouse rIL-4.
Ramezani-Rad P., Chen C., Zhu Z, & Rickert R.C. (2020). Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells. Cell reports, 33(7), 108403.
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