Szx10 dissecting microscope

Manufactured by Olympus

Sourced in Japan

The SZX10 is a dissecting microscope designed for detailed observation and examination of specimens. It features an optical system that provides a wide field of view and high magnification, allowing users to clearly see small details. The microscope is built with high-quality components and is suitable for a range of applications in various fields, including biology, zoology, and material science.

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Lab products found in correlation

Bx51 fluorescence microscope, by Olympus (3 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) Dp72 digital camera, by Olympus (1 mentions)

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SZX10 microscope Dissecting microscope SZX10

6 protocols using szx10 dissecting microscope

Microscopic Analysis of Spikelet and Anther

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The plants were imaged with a Nikon D7000 digital camera (Nikon, Japan) and an Olympus SZX10 dissecting microscope (Olympus, Japan). Images of spikelets and anthers were captured with an Olympus BX51 fluorescence microscope (Olympus, Japan). Pollen viability was assessed using Alexander’s solution for staining wild-type and mutant anthers. By the use of semi-thin sections along with SEM (JEOL, Japan) and TEM (Hitachi, Japan) observations, the spikelets and anthers of wild type and osms188 mutant plants were classified as belonging to different stages to avoid experimental deviation. The embedding and observation procedures were performed as described in a previous study (Lou et al. 2014 (link)).

Han Y., Zhou S.D., Fan J.J., Zhou L., Shi Q.S., Zhang Y.F., Liu X.L., Chen X., Zhu J, & Yang Z.N. (2021). OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice. Rice, 14, 4.

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Imaging of Transgenic Zebrafish Embryos

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Images of the embryos positive for the gene construct were screened with a BX51 fluorescence microscope (Olympus, Japan). Images of positive larvae were photographed with an FV1000 confocal microscope (Olympus) or BX51 fluorescence microscope (Olympus). Images of the immunohistochemistry were photographed with an FV1000 confocal microscope (Olympus). Whole-mount in situ hybridized larvae images were photographed with a DP72 digital camera mounted on an SZX10 dissecting microscope (Olympus, Japan). The resulting images were compiled in Adobe Photoshop CS2 (Adobe, San Jose, CA, USA) and resized. They were occasionally modified for contrast and brightness using the Image-Adjustments-Contrast-Brightness setting. All of the images within an experiment were manipulated similarly.

Fang Y., Lei X., Li X., Chen Y., Xu F., Feng X., Wei S, & Li Y. (2014). A novel model of demyelination and remyelination in a GFP-transgenic zebrafish. Biology Open, 4(1), 62-68.

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Assessing Cf-16-mediated Hypersensitive Response

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To assess the process of Cf-16-mediated HR and the key time points involved in the resistance mechanism, the lactophenol trypan blue staining method was performed according to Franco’s approach [64 (link)]. Leaf samples from the resistant and susceptible lines were harvested at 0–21 dpi, immediately stained, clarified overnight in chloral hydrate solution (2.5 mg/ml) [65 (link)], and examined using an Olympus SZX10 dissecting microscope (Olympus, Japan).

Zhang D., Bao Y., Sun Y., Yang H., Zhao T., Li H., Du C., Jiang J., Li J., Xie L, & Xu X. (2020). Comparative transcriptome analysis reveals the response mechanism of Cf-16-mediated resistance to Cladosporium fulvum infection in tomato. BMC Plant Biology, 20, 33.

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In Situ Hybridization for Tissue Samples

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In situ hybridization for whole tissues was carried out as described previously (Abler et al., 2011b ; Keil et al., 2012a (link)). For in situ hybridization on tissue sections, lower urinary tracts fixed overnight in phosphate buffered saline containing 4% paraformaldehyde were embedded in OCT embedding medium and cut into 10-micron sections before probe hybridization. Sequences for primers used in riboprobe synthesis are as follows: Mouse Nkx3–1 5’-CAGTGGCTGATGTCAAGG-3’ and 5’-CGATGTTAATACGACTCACTATAGGGCTAAGCAGGA AGGGCAGGAG-3’. After completion of the colorimetric reaction, tissues were fixed overnight in phosphate buffered saline containing 4% paraformaldehyde before imaging using a Nikon Eclipse 80i compound microscope or an Olympus SZX10 dissecting microscope.

Joseph D.B., Chandrashekar A.S., Abler L.L., Chu L.F., Thomson J.A, & Vezina C.M. (2019). Epithelial DNA methyltransferase-1 regulates cell survival, growth and maturation in developing prostatic buds. Developmental biology, 447(2), 157-169.

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Comprehensive Microscopy Techniques for Plant Phenotypic Analysis

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Photographs of plant phenotypes were taken with a Nikon D7000 digital camera (Nikon, Tokyo, Japan). Anthers were captured with an Olympus SZX10 dissecting microscope (Olympus, Tokyo, Japan). Pollen viability was assessed by I₂/KI staining and imaged with an Olympus BX51 fluorescence microscope (Olympus, Tokyo, Japan). The cytological observation of rice anthers was imaged by semi‐thin sectioning (Leica, Wetzlar, Germany), SEM (JEOL, Tokyo, Japan) and TEM (Hitachi, Tokyo, Japan). More detailed procedures for these analyses were described as previously reported (Han et al., 2021 (link)). The 4, 6‐diamino‐phenylindole staining of microspores was performed according to a previous study (Hong et al., 2012 (link)).

Han Y., Jiang S., Zhong X., Chen X., Ma C., Yang Y., Mao Y., Zhou S., Zhou L., Zhang Y., Huang X., Zhang H., Li L., Zhu J, & Yang Z. (2023). Low temperature compensates for defective tapetum initiation to restore the fertility of the novel TGMS line ostms15. Plant Biotechnology Journal, 21(8), 1659-1670.

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Nematode Length Measurement for SWCNT-COOH Effects

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The current study measured the lengths of nematodes to assess the effects of SWCNTs−COOH on growth. After exposure to the different concentrations of SWCNTs−COOH for 24 h, the nematodes were transferred to NGM plates with OP50 lawn and incubated for 48 h until the old L4 stage. An Olympus SZX10 dissecting microscope was used to obtain images, while ImageJ software [46 (link)] was used to measure the length of each of the nematodes. Three worms were measured for each concentration.

Lu J.H., Hou W.C., Tsai M.H., Chang Y.T, & Chao H.R. (2022). The Impact of Background-Level Carboxylated Single-Walled Carbon Nanotubes (SWCNTs−COOH) on Induced Toxicity in Caenorhabditis elegans and Human Cells. International Journal of Environmental Research and Public Health, 19(3), 1218.

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