Recombinant cd137l

Manufactured by Sangon

Recombinant CD137L is a laboratory reagent. It is a soluble, recombinant form of the CD137 ligand protein. CD137L is a member of the tumor necrosis factor superfamily and plays a role in immune system regulation.

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Lab products found in correlation

Tumor necrosis factor α, by Thermo Fisher Scientific (1 mentions) Tnf α, by Merck Group (1 mentions) 5 aza, by Merck Group (1 mentions) Tnf α, by Sangon (1 mentions) Recombinant murine vegf165, by Thermo Fisher Scientific (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Ly294002, by MedChemExpress (1 mentions)

2 protocols using recombinant cd137l

Isolation and Culture of Mouse Vascular Smooth Muscle Cells

Cited 1 time

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Mouse VSMCs were isolated from the aortas of male 8-week-old C57BL/6J mice as reported previously 18 (link), 19 (link). Cells were cultured in DF12 (Dulbecco’s modified Eagle’s medium and Ham’s F-12 nutrient mixture) containing 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37°C with 5% CO₂. Cells at <5 passages were used in all cell experiments. For related experiments, cells were treated with 10 ng/ml tumor necrosis factor (TNF)-α (PeproTech, Rocky Hill, New Jersey) for 24 h or TNF-α plus 5 μmol/ml of 5-aza (MilliporeSigma, St. Louis, Missouri) for 48 h. In a CD137-associated assay, VSMCs were pre-treated with TNF-α (10 ng/ml) for 24 h to elevate the CD137 levels on the membrane and further treated with 10 μg/ml recombinant CD137L (Sangon Biotech, Shanghai, China) to activate CD137 signaling (20) (link).

Zhong W., Li B., Xu Y., Yang P., Chen R., Wang Z., Shao C., Song J, & Yan J. (2018). Hypermethylation of the Micro-RNA 145 Promoter Is the Key Regulator for NLRP3 Inflammasome-Induced Activation and Plaque Formation. JACC: Basic to Translational Science, 3(5), 604-624.

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Exploring CD137 and VEGF in Atherosclerosis

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ApoE^−/− and ApoE^−/−CD137^−/− mice were fed with high fat diet until the 19th week. The following reagents were used in this study: TNFα (10 ng/mL, PeproTech), recombinant murine VEGF165 (20 ng/mL, PeproTech), recombinant CD137L (10 μg/mL, Sangon Biotech), human 4-1BB/TNFRSF9/CD137 antibody (10 μg/mL, R&D), XL184 (cabozantinib, an inhibitor of VEGFR2 which was applied to the tube formation assay and aortic ring assay, 0.03 μM, MedChemExpressan), LY294002 (an inhibitor of p-AKT, 5 μM, MedChemExpress), siRNA-CD137 (100 nM, GenePharma), siRNA-VEGFR2 (50 nM, GenePharma), CD137 (4-1BB) Monoclonal Antibody (10 μg/mL, eBioscience), and L-NG-nitroarginine Methyl Ester (L-NAME, eNOS inhibitor, 100 μM, MedChem Express).

Li B., Zhang Y., Yin R., Zhong W., Chen R, & Yan J. (2020). Activating CD137 Signaling Promotes Sprouting Angiogenesis via Increased VEGFA Secretion and the VEGFR2/Akt/eNOS Pathway. Mediators of Inflammation, 2020, 1649453.

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