Flow cytometry was implemented for measuring UC-MSC surface markers. Briefly, cells were incubated with FITC-conjugated primary antibodies after preprocessing steps as described in the section of cell cycle analysis. Then, the cells were subjected to a MACSQuant Analyzer Flow Cytometer (Miltenyi Biotec) for analysis. The primary antibodies used in this experiment are mouse anti-human CD34 (555821; BD Pharmingen), mouse anti-human CD45 (555482; BD Pharmingen), mouse anti-human CD73 (561254; BD Pharmingen), mouse anti-human CD90 (561969; BD Pharmingen), mouse anti-human CD105 (561443; BD Pharmingen), and mouse anti-human HLA-DR (555560; BD Pharmingen).
Mouse anti human cd105
Manufactured by BD
Sourced in United States
Mouse anti-human CD105 is a monoclonal antibody that binds to the CD105 antigen, also known as endoglin, which is a homodimeric transmembrane glycoprotein expressed on endothelial cells. This antibody can be used in various laboratory applications, such as flow cytometry and immunohistochemistry, to detect and study the expression of CD105.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti human cd34, by BD (3 mentions)
Mouse anti human cd45, by BD (3 mentions)
Mouse anti human hla dr, by BD (2 mentions)
Mouse anti human cd73, by BD (2 mentions)
Mouse anti human cd90, by BD (2 mentions)
Mouse anti human cd44, by BD (2 mentions)
Macsquant analyzer flow cytometer, by Miltenyi Biotec (1 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
Mouse anti human p75ntr, by BD (1 mentions)
3 protocols using mouse anti human cd105
1
Comprehensive Flow Cytometry Analysis of UC-MSC
2
Characterizing hiPSC-derived MSCs and rat BMSCs
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The hiPSC-derived MSCs or rat BMSCs were harvested using 0.25% trypsin/ethylenediaminetetraacetic acid and resuspended in 100 μl staining medium (2% FBS and 2% N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid in PBS). The cells were stained with mouse anti-human CD29 (BD, USA), mouse anti-human CD44 (BD, USA), mouse anti-human CD34 (BD, USA), mouse anti-human CD105 (BD, USA), and mouse anti-human CD45 (BD, USA) antibodies for 30 min at 4℃. Isotype-matched antibody (immuno-globulin G 2b-fluorescein isothiocyanate) was used to determine nonspecific fluorescence. Samples were run on a cytometer (CytoFLEX Beckman Coulter, USA).
3
Multiparametric Flow Cytometry Analysis of Stem Cell Markers
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Fourth‐passage cells (5 × 105) of each group were harvested and fixed with 4% paraformaldehyde for 30 minutes, followed by incubation with mouse anti‐human p75NTR, mouse anti‐human CD44, mouse anti‐human CD73, mouse anti‐human CD90, mouse anti‐human CD105, mouse anti‐human CD11b, mouse anti‐human CD19, mouse anti‐human CD34, mouse anti‐human CD45 and mouse anti‐human HLA‐DR antibodies (1:20; BD Pharmingen™) at 4°C for 2 hours. Subsequently, the specimens were analysed by flow cytometry.
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