2 aminoethyl methacrylate hydrochloride aema

Unless
otherwise noted, water was taken from
a Milli-Q source (Millipore) with 18.2 MΩ·cm resistivity,
referred to as MQ water. The monomers (see Figure 1) 2-aminoethyl methacrylate hydrochloride
(AEMA) and the deuterated methacrylic acid (dMAA) were obtained from
Sigma-Aldrich (St. Louis, MO) and Polymer Source Inc. (Montreal, Canada),
respectively. Ammonia, hydrogen peroxide, and sodium carbonate (AnalR
Normapure) were obtained from VWR (Stockholm, Sweden), and ethanol
was obtained from Solveco (Stockholm, Sweden). Tris(hydroxymethyl)aminomethane
was purchased from SERVA Electrophoresis GmbH (Heidelberg, Germany).
Glacial acetic acid was obtained from Merck (Darmstadt, Germany).
[3-(Methacryloyloxy)propyl]trimethoxysilane (MPS), sodium acetate,
sodium hydrogen phosphate, sodium dihydrogen phosphate, sodium bicarbonate,
and hydrochloric acid were from Sigma-Aldrich.

Atorvastatin calcium was from Biosynth (Compton, UK). 2-Hydroxyethyl methacrylate (HEMA) was supplied by Merck (Darmstadt, Germany). Ethylene glycol dimethacrylate (EGDMA), ethylene glycol phenyl ether methacrylate (EGPEM), 2,2′-azo-bis(isobutyronitrile) (AIBN), 2-aminoethyl methacrylate hydrochloride (AEMA), and dichlorodimethylsilane were from Sigma-Aldrich (Steinheim, Germany). N-(3-aminopropyl) methacrylamide hydrochloride (APMA) was from PolySciences Inc. (Warrington, PA, USA). Sodium chloride (NaCl) was from Scharlau (Barcelona, Spain), and ethanol absolute was from VWR Chemicals (Leuven, Belgium). Ultrapure water (resistivity > 18.2 MΩ cm; Milli-Q^®, Millipore Ibérica, Madrid, Spain) was obtained by reverse osmosis. Simulated lachrymal fluid (SLF) was prepared with the following composition in water: 6.78 g/L NaCl from Scharlau (Barcelona, Spain), 1.90 g/L NaHCO₃ from Probus (Barcelona, Spain), 1.38 g/L KCl, and 0.042 g/L CaCl₂ from Merck (Darmstadt, Germany) with pH 7.4 [23 (link)]. Carbonate buffer pH 7.2 was prepared by mixing two buffer solutions: buffer solution A (100 mL: 1.24 g NaCl, 0.071 g KCl, 0.02 g NaH₂PO₄ from Merck (Darmstadt, Germany), 0.49 g NaHCO₃) and buffer solution B (100 mL: 0.023 g CaCl₂, 0.031 g MgCl₂ from Panreac (Barcelona, Spain)). Balb/3T3 fibroblasts were provided by American Type Culture Collection (Manassas, VA, USA).