For DQ Ovalbumin measurements, microglia were treated with fibrils or monomer for 30 minutes followed by the addition of DQ Green BSA (Molecular Probes) for one hour. Anti-iNOS (Abcam) and anti-MHCII (clone M5/114.15.2, eBiosciences) were used to stain microglia as previously described (Harms 2013). Images were captured with a Lecia TCS-SP5 confocal microscope and intensities were quantified using ImageJ software. Conditioned media was collected from each experiment and supernatants analyze with the Milliplex Mouse Cytokine/Chemokine Magnetic Bead 25 Plex Kit (MD Millipore)
Anti mhc 2 clone m5 114
The Anti–MHC II clone M5/114.15.2 is a monoclonal antibody that recognizes the major histocompatibility complex (MHC) class II molecules. The primary function of this product is to serve as a tool for the identification and characterization of MHC class II-expressing cells in research applications.
Lab products found in correlation
4 protocols using anti mhc 2 clone m5 114
Isolation and Characterization of Primary Microglia
For DQ Ovalbumin measurements, microglia were treated with fibrils or monomer for 30 minutes followed by the addition of DQ Green BSA (Molecular Probes) for one hour. Anti-iNOS (Abcam) and anti-MHCII (clone M5/114.15.2, eBiosciences) were used to stain microglia as previously described (Harms 2013). Images were captured with a Lecia TCS-SP5 confocal microscope and intensities were quantified using ImageJ software. Conditioned media was collected from each experiment and supernatants analyze with the Milliplex Mouse Cytokine/Chemokine Magnetic Bead 25 Plex Kit (MD Millipore)
Thymic Epithelial Cell Isolation and Analysis
Isolation and Flow Cytometry Analysis of Thymic Epithelial Cells
Isolation and Flow Cytometry Analysis of Thymic Epithelial Cells
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