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Ab77609

Manufactured by Abcam
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Ab77609 is a laboratory product offered by Abcam. It is a piece of equipment designed for use in scientific research and experiments. The core function of this product is to provide a tool for researchers to conduct their investigations. No further details about the intended use or specific capabilities of this product are provided.

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Lab products found in correlation

Ab 11333, by Abcam (1 mentions)

2 protocols using ab77609

1

Immunohistochemical Detection of HSP47

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After deparaffinization, the slides were treated in incubator at 60oC for 30 min. Specimens were subjected to proteolytic enzyme (Protease, Nichirei), immersed in 0.03% hydrogen peroxide methanol solution for 3 min, followed by endogenous peroxidase activation for 10 min. Anti-HSP47 (ab77609, Abcam) diluted at 2000x was the primary antibody. Polyclonal anti-rabbit (Simple Stain, Mouse MAX-PO ®, Tokyo, Nichirei) was the secondary antibody. Then after, slides were washed with PBS and then subjected to DAB color development for 3 min. Finally, counterstaining was done by immersing the specimen in hematoxylin for 1 sec.
Mimura H., Takaya T., Matsuda S., Nakano K., Muraoka R., Tomida M., Okafuji N., Fujii T, & Kawakami T. (2016). Functional Role of HSP47 in the Periodontal Ligament Subjected to Occlusal Overload in Mice. International Journal of Medical Sciences, 13(4), 248-254.
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2

Isolation of Fibroblasts from EBs

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Once EBs were plated after 4 days of suspension culture, spontaneous fibroblasts were allowed to grow out and visually assessed. The fibroblasts were manually dissected from the culture plate and stained for HSP47 (catalog no. AB77609, Abcam) and fibroblast surface protein (FSP) (catalog no. AB11333, Abcam). The cell populations that were positive for both markers were used for further studies.
Sequiera G.L., Srivastava A., Sareen N., Yan W., Alagarsamy K.N., Verma E., Aghanoori M.R., Aliani M., Kumar A., Fernyhough P., Rockman-Greenberg C, & Dhingra S. (2022). Development of iPSC-based clinical trial selection platform for patients with ultrarare diseases. Science Advances, 8(14), eabl4370.
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