Irdye 800cw anti rabbit igg h l

Western blotting and immunofluorescence were performed as previously described [46 ]. The antibodies used were: rabbit polyclonal anti-phospho-DNA-PKcs (Ser2056) (4215, Cell Signaling), mouse monoclonal anti-phospho-H2A.X (Ser139) (05-636, Millipore), and mouse monoclonal anti-alpha-Tubulin (T9026, Sigma-Aldrich). As secondary antibodies for Western blotting, goat IRDye 680RD anti-mouse IgG (H+L) (926-68070, Li-COR Bioscience), and IRDye 800CW anti-rabbit IgG (H+L) (926-32211, Li-COR Bioscience) were used. Results were visualized using an Odyssey Infrared Imaging System (Li-COR Bioscience). Alexa Fluor 488 goat anti-mouse IgG (A11001, Life Technologies) was used as a secondary antibody for immunofluorescence.

In this experiment, CCl₄ was purchased from Jiangsu Qiangsheng Chemical (Jiangsu, China). The positive drug Bicyclol was obtained from Beijing union pharmaceutical factory (Beijing, China). The detection kits for superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), and malondialdehyde (MDA) were all purchased from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). Enzyme-linked immunosorbent assay kits for mouse TNF-a were purchased from R&D Systems (Minneapolis, USA). Monoclonal anti-NF-κB rabbit antibody was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Monoclonal anti-Bcl-2, anti-Bax, anti-cleaved caspase-3, and GAPDH antibodies were obtained from Cell Signaling Technology (Danvers, MA). In addition, antibodies against JNK, p-JNK, and p-NF-κB were also from Cell Signaling Technology (Danvers, MA). CYP2E1 antibodies were purchased from Sangon Biotechnology (Shanghai, China). Secondary antibodies used in Western blot were IRDye® 800CW anti-rabbit IgG (H+L) (LI-COR Biosciences) and IRDye 800CW anti-mouse IgG (H+L) (LI-COR Biosciences).