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2 protocols using anti bcl2 l 13

1

Antibody Characterization for Cell Biology

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Anti-Tim23 (1/1,000), anti-Tom20 (for western blots, 1/1,000, for immunofluorescence, 1/200), anti-Bcl2-L-13 (1/1,000) (Proteintech), anti-cytochrome c (1/1,000) (BD biosciences), anti-Bcl2-L-13 (1/500), anti-GAPDH (1/1,000), anti-Fis1 (1/1,000), anti-α-tubulin (1/1,000) (Abcam), anti-LC3B (for western blots, 1/1,000, for immunofluorescence, 1/100), anti-HA (for western blots, 1/1,000, for immunofluorescence, 1/200), anti-Drp1Ser637 (1/500), anti-Drp1Ser616 (1/1,000), anti-Mfn2 (1/1,000), anti-cleaved caspase 3 (1/1,000) (Cell Signaling Technology), anti-ATP synthase subunit beta (for western blots, 1/1,000, for immunofluorescence, 1/200) (Life Technologies), anti-Drp1 (for western blots, 1/1,000, for immunofluorescence, 1/100), anti-Opa1 (1/1,000) (BD Transduction Laboratories), anti-FLAG (for immunofluorescence, 1/200) (Sigma), anti-Mfn1 (1/1,000) (Abnova), anti-ubiquitin (for immunofluorescence, 1/200) (Enzo Life Science), anti-Phospho-Serine/Threonine (Upstate), anti-mCherry (1/500) (Clontech) and anti-RFP23 (link) (1/1,000) antibodies were used in this study.
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2

Mitochondrial Dynamics and Apoptosis Regulation

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Anti-Tim23 (1/1000), anti-Tom20 (for western blots, 1/1000, for immunofluorescence, 1/200), anti-Bcl2-L-13 (1/1000) (Proteintech), anti-cytochrome c (1/1000) (BD biosciences), anti-Bcl2-L-13 (1/500), anti-GAPDH (1/1000), anti-Fis1 (1/1000), anti-α-tubulin (1/1000) (Abcam), anti-LC3B (for western blots, 1/1000, for immunofluorescence, 1/100), anti-HA (for western blots, 1/1000, for immunofluorescence, 1/200), anti-Drp1Ser637 (1/500), anti-Drp1Ser616 (1/1000), anti-Mfn2 (1/1000), anti-cleaved caspase 3 (1/1000) (Cell Signalling Technology), anti-ATP synthase subunit beta (for western blots, 1/1000, for immunofluorescence, 1/200) (Life Technologies), anti-Drp1 (for western blots, 1/1000, for immunofluorescence, 1/100), anti-Opa1 (1/1000) (BD Transduction Laboratories), anti-FLAG (for immunofluorescence, 1/200) (Sigma), anti-Mfn1 (1/1000) (Abnova), anti-ubiquitin (for immunofluorescence, 1/200) (Enzo Life Science), anti-Phospho-Serine/Threonine (Upstate), anti-mCherry (1/500) (Clontech) and anti-RFP23 (link) (1/1000) antibodies were used in this study.
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