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Rat lh elisa kit

Manufactured by Cusabio
Sourced in China, United States

The Rat LH ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of luteinizing hormone (LH) levels in rat serum or plasma samples. The kit utilizes specific antibodies and enzyme-linked immunosorbent assay (ELISA) technology to detect and quantify the target analyte.

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2 protocols using rat lh elisa kit

1

Measurement of Serum Hormones in Rats

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Blood samples were collected directly from the inferior vena cava using a 1-mL syringe at the end of the experiment. Serum was obtained by centrifugation at 2000× g for 10 min and stored at −70 °C until use. Serum LH levels were measured using a rat LH ELISA kit (Cusabio Biotech, Wuhan, China). Serum testosterone levels were measured using a testosterone ELISA kit (Abcam, Cambridge, UK). 17β-Estradiol (E2) levels were measured using an estradiol (rat) ELISA kit (BioVision, Mountain View, CA, USA). All kits were used according to the manufacturers’ instructions.
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2

Hormonal Profile Analysis in Rats

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The serum levels of estradiol (E2), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone (T) were determined using ELISA kits: Rat Estradiol ELISA Kit (My BioSource, USA, Cat# MBS843353), Rat LH ELISA Kit (CUSABIO Biotech Co., Ltd., China, Cat# CSB-E12654r), Rat FSH ELISA Kit (CUSABIO Biotech Co., Ltd., China, Cat# CSB-E06869r), and Mouse/Rat Testosterone ELISA Kit (Sigma Aldrich, USA, Cat# SE120089) respectively according to the protocol provided with each kit. In addition, the LH/FSH ratio was measured.
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