Rabbit anti human parg igg ab16060

HeLa cells were provided by Riken (Tsukuba, Japan) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum, penicillin (100 U/ml), and streptomycin (100 μg/ml), in a humidified incubator with 5% CO₂ at 37 °C. 3-Aminobenzamide (3AB) was obtained from Tokyo Chemical Industry (Tokyo, Japan). Olaparib was purchased from ChemScene (NJ, USA), emetine hydrochloride from Cayman Chemical (No. 21048; MI, USA), and PDD00017273 [37 (link)] from Tocris Bioscience (MN, USA), and PD0325901 from ChemScene. The following antibodies were obtained from Santa Cruz Biotechnology (TX, USA): mouse anti-human PARP1 IgG (sc-8007), mouse anti-human phosphorylated ERK IgG (sc-7383), mouse anti-human ERK2 IgG (sc-1647), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-2005), and HRP-conjugated goat anti-rabbit IgG (sc-2004). Rabbit anti-human PARG IgG (ab16060) was purchased from Abcam (Cambridge, UK). Anti-PAR (10H, IgG3 kappa), secreted from hybridoma cells [38 (link)], was purified using a Protein A Sepharose Fast Flow column (GE Healthcare, IL, USA). Anti-PAR polyclonal antibodies were produced in rabbits by injecting PAR mixed with methylated BSA in our laboratory [39 (link),40 ]; the antibodies were purified with a Protein A Sepharose Fast Flow column.