Waters 2695 hplc instrument

Manufactured by Waters Corporation

Sourced in United States, China

The Waters 2695 HPLC instrument is a high-performance liquid chromatography system designed for analytical and preparative separations. It features a quaternary solvent delivery system, an autosampler, and a temperature-controlled column compartment. The 2695 HPLC instrument is capable of precise and reproducible separation of complex mixtures.

Automatically generated - may contain errors

Lab products found in correlation

Geniposidic acid, by Yuanye Bio-Technology (1 mentions) Geniposide, by Yuanye Bio-Technology (1 mentions) Genipin 1 gentiobioside, by Yuanye Bio-Technology (1 mentions) Sunfire c18 column, by Waters Corporation (1 mentions) Empower software, by Waters Corporation (1 mentions) Blue dextran, by Merck Group (1 mentions)

Close spelling variants of this product

Waters 2695 HPLC 2695 HPLC instrument Waters HPLC instrument Waters 2695 2695 HPLC HPLC instrument

5 protocols using waters 2695 hplc instrument

HPLC Analysis of Isoliquiritigenin

Check if the same lab product or an alternative is used in the 5 most similar protocols

For this study, we utilized a Kq-400 db ultrasonic cleaner from Shenzhen Keweida Ultrasonic Equipment Co. Ltd. (Shenzhen, China), a 98-1-b electronic temperature-regulating heating sleeve from Heze Shengbang Instrument Development Co. Ltd. (Heze, China), a tp-213 electronic balance from Shanghai Ziyi Instrument Equipment Co. Ltd. (Shanghai, China), and a Waters 2695 HPLC instrument from Waters Co. (Milford, MA, USA). In addition, a HiQ Sil-C18 reversed-phase column (4.6 mm × 250 mm, 5 μm, KYA TECH Corp., Tokyo, Japan) was used for chromatographic separation.
We utilized an acetonitrile-water-acetic acid (32 : 68 : 0.5, v/v/v) mobile phase for HPLC analyses, with a 1.0 mL/min flow rate, a 10 μL injection volume, and a column temperature at 25°C column temperature. Isoliquiritigenin was then detected based on absorbance at 350 nm (see Figure 1).
The resultant calibration curve used the formula (Y = 3 × 10⁷) (X + 2 × 10⁶) (R² = 0.9911, X: isoliquiritigenin concentration, Y: peak area), indicating a good linear fit for isoliquiritigenin.

Hao J., Liu J., Zhang L., Jing Y, & Ji Y. (2020). A Study of the Ionic Liquid-Based Ultrasonic-Assisted Extraction of Isoliquiritigenin from Glycyrrhiza uralensis. BioMed Research International, 2020, 7102046.

+ Open protocol

+ Expand

HPLC Analysis of Iridoid Glycosides

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HPLC separation was performed using a Waters WATO54275-C18 column (250x4.6 mm, 5 µm, Waters China, Ltd.) installed on a Waters 2695 HPLC instrument (Waters Corporation). The mobile phase consisted of 0.1% phosphoric acid (A) and acetonitrile (B) with a gradient elution: 0-15 min, 4-25% B; 15-20 min, 25-28% B; 20-40 min, 28-38% B; 40-41 min, 95% B; 41-51 min, 95-4% B. The flow rate was 1 ml/min, and the injection volume was 10 µl. The temperature of the column was set at 25˚C. Standard compounds, including geniposidic acid, deacetylasperulosidic acid, genipin-1-gentiobioside, geniposide, crocin I and II with >98% purity were purchased from Shanghai Yuanye Bio-Technology Co., Ltd.

Chen J., Tchivelekete G.M., Zhou X., Tang W., Liu F., Liu M., Zhao C., Shu X, & Zeng Z. (2021). Anti-inflammatory activities of Gardenia jasminoides extracts in retinal pigment epithelial cells and zebrafish embryos. Experimental and Therapeutic Medicine, 22(1), 700.

+ Open protocol

+ Expand

HPLC Analysis of XJDH Constituents

Check if the same lab product or an alternative is used in the 5 most similar protocols

For HPLC analysis, XJDH was filtered through a 0.45 μm membrane filter before injection. HPLC using Waters 2695 HPLC instrument (Waters Co., Milford, MA, USA) was performed on a SunFire™ C18 column (5 μm, 250 mm × 4.6 mm i.d., Waters Co., Milford, MA, USA) with a mobile phase gradient of acetonitrile-water (2% to 100%) for 70 min. The injection volume was 10 μL of sample and mobile phase flow rate was set at 1 mL/min with UV detection at 254 nm. Acquisition and analysis of chromatographic data were performed using Empower software (Waters Co., Milford, USA). Stock solutions of paeoniflorin, albiflorin, and oxypaeoniflorin were prepared for quantification of XJDH. The concentrations of major constituents were determined by regression equations, calculated in the form of y = ax + b, where y and x were peak area and contents of the compound, respectively.

Zhang X., Fei X., Tao W., Li J., Shen H., Wang X., Liu H, & Xu Y. (2017). Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88/NF-κB Signaling Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 3848595.

+ Open protocol

+ Expand

Gel Permeation Chromatography for Molecular Weight

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chromatographic system consisted of a Waters 2695 HPLC instrument (Waters Corp., Milford, USA). equipped with a quaternary pump, a degasser, an autosampler, a photodiode-array detector with a 13 μL flow cell, and software for data acquisition and analysis. The chromatographic column used for separation at room temperature was PSS Suprema Analytical Lineal S 100−100.000 Da (PSS Polymer Standards Service GmbH, Mainz, Germany). The composition of the mobile phase was 300 mM KCl and 20 mM phosphate (pH 7.1). The flow was set to 0.8 mL·min⁻¹. The injection volume was 15 μL. T₁₅, T₂₀, T₂₅, T₂₀ and T₄₅ sequences were used as standards to construct the plot of logarithm of the retention time (t_R) vs. molecular weight [37 (link)]. Blue dextran (MW 2,000,000 Da, Sigma-Merck, Darmstadt, Germany) was used as a void volume marker (5.30 mL).

Mazzini S., Gargallo R., Musso L., De Santis F., Aviñó A., Scaglioni L., Eritja R., Di Nicola M., Zunino F., Amatulli A, & Dallavalle S. (2019). Stabilization of c-KIT G-Quadruplex DNA Structures by the RNA Polymerase I Inhibitors BMH-21 and BA-41. International Journal of Molecular Sciences, 20(19), 4927.

+ Open protocol

+ Expand

Extraction and Characterization of AC591 Phytochemicals

Check if the same lab product or an alternative is used in the 5 most similar protocols

To prepare AC591 samples, AR, CR, PRA, JF, and ZR were mixed at a ratio of 2:1:1:1:1 and refluxed for 2 h with 10 volumes of water after maceration for 30 min. The water extract was filtered, and the residue refluxed again with 8 volumes of water for 2 h. The combined filtrates were desiccated to produce the AC591 extract at a concentration of 20 g crude drug/mL, and stored at -80°C. The extracts were filtered through a 0.2 μm PTFE syringe filter.
Qualitative analysis was performed by HPLC-MS/MS system consisting of Waters 2695 HPLC instrument and Quattro Premier XE MircoMass triple quadrupole tandem mass spectrometer (Waters Co., Milford, MA, United States). Chromatographic separation was carried out at 35°C on a Waters MS C₁₈ column (250 mm × 4.6 mm, 5.0 μm) using the following gradient profile: 5–15% B (0–0.15 min), 15–20% B (15–35 min), 20–40% B (35–65 min), 40–60% B (65–75 min), 60–100% B (75–90 min). The mobile phase was composed of 0.1% acetic acid (A) and 100% acetonitrile (B). The injection volume was 2 μL. Quantification analysis was carried out using HPLC-DAD method. The concentrations of major constituents were determined by an external standard curve.

Cheng X., Huo J., Wang D., Cai X., Sun X., Lu W., Yang Y., Hu C., Wang X, & Cao P. (2017). Herbal Medicine AC591 Prevents Oxaliplatin-Induced Peripheral Neuropathy in Animal Model and Cancer Patients. Frontiers in Pharmacology, 8, 344.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!