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Infinity alt kit

Manufactured by Thermo Fisher Scientific
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The Infinity ALT kit is a laboratory assay designed to quantitatively measure the activity of the enzyme Alanine Aminotransferase (ALT) in biological samples. This kit provides the necessary reagents and protocols to perform the ALT activity assay in a laboratory setting.

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Lab products found in correlation

Triglyceride liquid reagents kit, by Horiba (16 mentions) Ethanol assay kit, by Abcam (14 mentions) Mouse total bile acid kit, by Crystal Chem (2 mentions) Elisa, by Cusabio (2 mentions) Proinflammatory panel 1 mouse kit, by Mesoscale (1 mentions) Ifabp elisa kit, by MyBioSource (1 mentions) Human zonulin elisa kit, by MyBioSource (1 mentions) Cholesterol liquid reagents kit, by Horiba (1 mentions) Wallac victor 1420 multilabel counter, by PerkinElmer (1 mentions) Mouse albumin elisa kit e99 134, by Fortis Life Sciences (1 mentions) Ceb526ge, by Cloud-Clone (1 mentions) Oil red o staining, by Merck Group (1 mentions) Quantichrom alkaline phosphatase assay kit, by BioAssay Systems (1 mentions) Elisa kit, by Fortis Life Sciences (1 mentions) Mouse il 22 duoset elisa kit, by R&D Systems (1 mentions)

20 protocols using infinity alt kit

1

Biochemical Profiling of Liver Function

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Serum levels of ALT were determined using Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using Triglyceride Liquid Reagents kit (Pointe Scientific). Levels of serum LPS and fecal albumin were determined by ELISA kits (Lifeome Biolabs and Bethyl Labs, respectively). Serum levels of ethanol were measured using Ethanol Assay kit (BioVision).
Duan Y., Llorente C., Lang S., Brandl K., Chu H., Jiang L., White R.C., Clarke T.H., Nguyen K., Torralba M., Shao Y., Liu J., Hernandez-Morales A., Lessor L., Rahman I.R., Miyamoto Y., Ly M., Gao B., Sun W., Kiesel R., Hutmacher F., Lee S., Ventura-Cots M., Bosques-Padilla F., Verna E.C., Abraldes J.G., Brown RS J.r., Vargas V., Altamirano J., Caballería J., Shawcross D.L., Ho S.B., Louvet A., Lucey M.R., Mathurin P., Garcia-Tsao G., Bataller R., Tu X.M., Eckmann L., van der Donk W.A., Young R., Lawley T.D., Stärkel P., Pride D., Fouts D.E, & Schnabl B. (2019). Bacteriophage targeting of gut bacterium attenuates alcoholic liver disease. Nature, 575(7783), 505-511.
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2

Biochemical Profiling of Liver Function

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Serum levels of ALT were determined using Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using Triglyceride Liquid Reagents kit (Pointe Scientific). Levels of serum LPS and fecal albumin were determined by ELISA kits (Lifeome Biolabs and Bethyl Labs, respectively). Serum levels of ethanol were measured using Ethanol Assay kit (BioVision).
Duan Y., Llorente C., Lang S., Brandl K., Chu H., Jiang L., White R.C., Clarke T.H., Nguyen K., Torralba M., Shao Y., Liu J., Hernandez-Morales A., Lessor L., Rahman I.R., Miyamoto Y., Ly M., Gao B., Sun W., Kiesel R., Hutmacher F., Lee S., Ventura-Cots M., Bosques-Padilla F., Verna E.C., Abraldes J.G., Brown RS J.r., Vargas V., Altamirano J., Caballería J., Shawcross D.L., Ho S.B., Louvet A., Lucey M.R., Mathurin P., Garcia-Tsao G., Bataller R., Tu X.M., Eckmann L., van der Donk W.A., Young R., Lawley T.D., Stärkel P., Pride D., Fouts D.E, & Schnabl B. (2019). Bacteriophage targeting of gut bacterium attenuates alcoholic liver disease. Nature, 575(7783), 505-511.
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3

Biochemical Markers of Hepatic Injury

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Serum levels of ALT were determined using Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using Triglyceride Liquid Reagents kit (Pointe Scientific). Serum levels of ethanol were measured using Ethanol Assay kit (BioVision). Serum levels of cytokines were measured using Proinflammatory Panel 1 (mouse) kit (Meso Scale Diagnostics). All results were measured using the software SoftMax Pro 7.0.3.
Duan Y., Chu H., Brandl K., Jiang L., Zeng S., Meshgin N., Papachristoforou E., Argemi J., Mendes B.G., Wang Y., Su H., Sun W., Llorente C., Hendrikx T., Liu X., Hosseini M., Kisseleva T., Brenner D.A., Bataller R., Ramachandran P., Karin M., Fu W, & Schnabl B. (2021). CRIg on liver macrophages clears pathobionts and protects against alcoholic liver disease. Nature Communications, 12, 7172.
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4

Evaluating Liver and Intestinal Markers

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Serum levels of ALT were measured using the Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific). Serum levels of ethanol were measured using the Ethanol Assay Kit (BioVision). Serum levels of intestinal fatty-acid binding protein (IFABP) were measured using the iFABP ELISA kit (MyBioSource). Serum levels of zonulin were measured using the Human Zonulin ELISA Kit (MyBioSource)17 .
Chu H., Duan Y., Lang S., Jiang L., Wang Y., Llorente C., Liu J., Mogavero S., Bosques-Padilla F., Abraldes J.G., Vargas V., Tu X.M., Yang L., Hou X., Hube B., Stärkel P, & Schnabl B. (2019). The Candida albicans exotoxin Candidalysin promotes alcohol-associated liver disease. Journal of hepatology, 72(3), 391-400.
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5

Quantifying Liver and Bile Metabolites

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Levels of plasma ALT were measured using the Infinity ALT kit (Thermo Scientific, Waltham, MA). Plasma and hepatic triglyceride and cholesterol levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific, Canton, MI) and Cholesterol Liquid Reagents kit (Pointe Scientific), respectively. Plasma levels of leptin were measured using the mouse leptin enzyme-linked immunosorbent assay kit according to the manufacturer’s protocol (Crystal Chem, Elk Grove Village, IL). Total bile acids and the bile acid pool were quantified using a Mouse Total Bile Acid Kit (Crystal Chem) as described.59 (link) For the total bile acid pool, total liver bile acids, total gallbladder bile acids, and total bile acids from the small intestine and cecum, contents were measured and calculated per gram of body weight.
Zhou R., Llorente C., Cao J., Zaramela L.S., Zeng S., Gao B., Li S.Z., Welch R.D., Huang F.Q., Qi L.W., Pan C., Huang Y., Zhou P., Beussen I., Zhang Y., Bryam G., Fiehn O., Wang L., Liu E.H., Yu R.T., Downes M., Evans R.M., Goglin K., Fouts D.E., Brenner D.A., Bode L., Fan X., Zengler K, & Schnabl B. (2021). Intestinal α1-2-Fucosylation Contributes to Obesity and Steatohepatitis in Mice. Cellular and Molecular Gastroenterology and Hepatology, 12(1), 293-320.
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6

Comprehensive Metabolic Assessment in Mouse Models

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Plasma levels of ethanol were measured using the Ethanol Assay Kit (BioVision). Levels of ALT were measured using Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific). Hepatic alcohol dehydrogenase (ADH) activity was measured using the ADH Assay Kit (BioVision). Plasma LPS (Cloud-Clone Corp), and fecal albumin levels (Bethyl lab) were measured by ELISA. Whole blood was collected into potassium EDTA–containing Microvette 100 tubes (Sarstedt), and automated complete blood counts (CBCs) were obtained using a Scil Vet abc automatic hematology analyzer (Scil Animal Care). For insulin tolerance test (ITT), following a 6-hour fasting period, an intraperitoneal dose of 0.35 or 1 U kg−1 insulin (novolin, Novo Nordisk Inc.) was administered to mice on RC diet or HFD, respectively, and blood glucose was measured at indicated time points. Plasma and liver levels of iron were measured using a kit from Thermo Scientific.
Llorente C., Jepsen P., Inamine T., Wang L., Bluemel S., Wang H.J., Loomba R., Bajaj J.S., Schubert M.L., Sikaroodi M., Gillevet P.M., Xu J., Kisseleva T., Ho S.B., DePew J., Du X., Sørensen H.T., Vilstrup H., Nelson K.E., Brenner D.A., Fouts D.E, & Schnabl B. (2017). Gastric acid suppression promotes alcoholic liver disease by inducing overgrowth of intestinal Enterococcus. Nature Communications, 8, 837.
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7

Serum Liver Enzyme Measurement

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Venous blood of mice was taken from the tail vein at different time points after APAP treatment. Serum ALT and AST levels were measured using the Infinity ALT Kit (Thermo Scientific) and AST Kit (Bioo Scientific), respectively. Briefly, 10-μL serum was added into 100-μL ALT or AST reagent, followed by reading of 340 nm at 37°C using a Wallac Victor 1420 Multilabel Counter microplate reader (PerkinElmer). Each sample was measured in triplicate.
Chen D., Ni H.M., Wang L., Ma X., Yu J., Ding W.X, & Zhang L. (2019). PUMA induction mediates acetaminophen-induced necrosis and liver injury. Hepatology (Baltimore, Md.), 69(5), 2164-2179.
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8

Plasma Biomarker Quantification

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Levels of plasma alanine amino-transferase (ALT) were measured using infinity ALT kit (Thermo Fisher Scientific, Waltham, MA). Triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific, Canton, MI). Levels of plasma ethanol were measured using the Ethanol Assay Kit (Bio Vision, Milpitas, CA).
Zhou R., Llorente C., Cao J., Gao B., Duan Y., Jiang L., Wang Y., Kumar V., Stärkel P., Bode L., Fan X, & Schnabl B. (2020). Deficiency of Intestinal α1-2-fucosylation Exacerbates Ethanol-Induced Liver Disease in Mice. Alcoholism, clinical and experimental research, 44(9), 1842-1851.
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9

Fecal Albumin and Liver Biomarkers

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Fecal albumin levels were measured by ELISA (Mouse Albumin ELISA kit E99-134, BETHYL). The plasma levels of alanine aminotransferase (ALT) and hepatic levels of triglyceride were measured using the Infinity ALT kit (Thermo Fisher Scientific) and Triglyceride Liquid Reagents Set (T7532-120, Pointe Scientific), respectively. Plasma lipopolysaccharide (LPS) levels were measured by ELISA (CEB526Ge; Cloud-Clone Corp.).
Yang A.M., Lin C.Y., Liu S.H., Syu G.D., Sun H.J., Lee K.C., Lin H.C, & Hou M.C. (2022). Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis. Frontiers in Microbiology, 13, 887728.
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10

Serum ALT Measurement in DEN-Treated Mice

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Venous blood of mice was collected from the tail vein 0, 24, and 72 h after DEN treatment. Blood was kept at 4 °C for overnight and centrifuged at 200 × g for 20 min to isolate serum. ALT was measured using the Infinity™ ALT kit (Thermo Scientific, Middletown, VA) and is reported as mean ± SD. Briefly, 10 μl of serum was added to 100 μl ALT reagent and measured at 37 °C at 340 nm. Each sample was measured in triplicate and three mice were used in each group.
Shang N., Bank T., Ding X., Breslin P., Li J., Shi B, & Qiu W. (2018). Caspase-3 suppresses diethylnitrosamine-induced hepatocyte death, compensatory proliferation and hepatocarcinogenesis through inhibiting p38 activation. Cell Death & Disease, 9(5), 558.
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