Goat anti rat secondary antibody

RIPK3 (1:2000 cat# NBP1-77299, Novus Biologicals, Centennial, CO, USA), internal control GAPDH (1:100,000, Cat# MAB374, Sigma-Aldrich, St Louis, MO, USA), β-ACTIN (1:2000, Cat# 4967, Cell Signaling Technology, Inc., Danvers, MA, USA), RIPK1(1:1000, Cat# MAB3585, R&D systems, Inc., Minneapolis, MN, USA), HIF1α (1:1000, Cat# 3716, Cell Signaling Technology, Inc., Danvers, MA, USA), cleaved CASPASE3 (1:5000, Cat# 9664, Cell Signaling Technology, Inc., Danvers, MA, USA), MLKL (1:2000, Cat# MABC604, Millipore Corp, Burlington, MA, USA), CASPASE1 (1:100, Cat# sc-56036, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and CASPASE8 (1:100, Cat# sc-81656, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) primary antibodies were used for the study. Horseradish peroxidase (HRP) conjugated goat anti-rabbit secondary antibody (Cat# 656120, Invitrogen, Thermo Fisher Scientific, Hanover Park, IL, USA) was used for cleaved CASPASE3 (1:100,000), RIPK3, HIF1α, CASPASE8, CASPASE1 (1:20,000), goat anti-rat secondary antibody (Cat# 629320, Invitrogen, Thermo Fisher Scientific, IL, USA) was used for MLKL (1:20000) and goat anti-mouse secondary antibody (Cat# G21040, Invitrogen, Thermo Fisher Scientific, IL, USA) was used for RIPK1 (1:20,000) and GAPDH (1:100,000).