2 20 diazobis 2 aminodinopropane dihydrochloride

Gallic acid (GA), Folin–Ciocalteu (FC) reagent, 6-hydroxy-2,5,7,8-tetramethyl-2-carboxylic acid (Trolox), fluorescein, 2,20-diazobis-(2-aminodinopropane)-dihydrochloride (AAPH), 2,20-azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS^•+), 2,2-diphenyl-1-picrylhydrazyl (DPPH), iron(III) chloride hexahydrate (FeCl₃∙6H₂O), 2,4,6-tripyridyl-triazine (TPTZ), iron(II) sulfate heptahydrate (FeSO₄∙7H₂O), Gallic acid, apigenin, ferulic acid, avenanthramide C, p-coumaric acid and sinapic acid were obtained from Sigma-Aldrich, Co. (St. Louis, MO, USA). Amyloglucosidase (EC 3.2.1.3) and glucose oxidase-peroxidase (GOPOD) were provided by Megazyme (Wicklow, Ireland). Glacial acetic acid, sodium acetate and chlorhydric acid were obtained from PanReac AppliChem (ITW Reagents, Darmstadt, Germany). The solvents were HPLC-grade (Sigma Aldrich Co., Madrid, Spain, and Merck KGaA, Darmstadt, Germany). Food-grade enzymes UltraFlo XL and Viscoferm were kindly provided by Novozymes (Bagsværd, Copenhagen, Denmark).

Fluorescein, 6-hydroxy-2,5,7,8-tetramethyl-2-carboxylic acid (Trolox), 2,20-diazobis-(2-aminodinopropane)-dihydrochloride (AAPH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,20-azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS^•+), Folin–Ciocalteu (FC) reagent, gallic acid (GA), iron (III) chloride hexahydrate (FeCl₃∙6H₂O), iron (II) sulfate heptahydrate (FeSO₄∙7H₂O), 2,4,6-tripyridyl-triazine (TPTZ), ferulic acid, hidroxybenzoic acid and p-coumaric acid were obtained from Sigma-Aldrich, Co. (St. Louis, MO, USA). Glucose oxidase-peroxidase (GOPOD) was supplied by Megazyme (Wicklow, Ireland). Sodium acetate, hydrochloric acid, glacial acetic acid and formic acid were purchased from PanReac AppliChem (ITW Reagents, Darmstadt, Germany). Different solvents were HPLC grade (Merck KGaA, Darmstadt, Germany, and Sigma Aldrich Co., Madrid, Spain). Viscoferm and UltraFlo XL food-grade enzymes were kindly supplied by Novozymes (Bagsværd, Copenhagen, Denmark).