For conditioned medium (Con medium), DMEM was incubated with SSCs of 90% confluence for 48 h, passed through a 0.22 mm filter, put in Amicon Ultra-15 Centrifugal Filter Unites (Millipore, Billerica, MA) with a cut-off of 3000 dalton Nominal Molecular Weight Limit, and centrifuged at 4000 ×g for 40 minutes.
Amicon ultra 15 centrifugal filter unites
Manufactured by Merck Group
The Amicon Ultra-15 Centrifugal Filter Units are laboratory equipment designed for the concentration and purification of biological samples. They utilize a centrifugal force to pass the sample through a semipermeable membrane, allowing for the separation of molecules based on their size.
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Lab products found in correlation
2 protocols using amicon ultra 15 centrifugal filter unites
1
Isolation of Conditioned Medium from SSCs
2
MSC-Derived Secreted Factors Isolation
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DMEM was incubated with 90% confluent PD-MSCs for 48 h to collect secreted factors made by MSC. Protein fraction of the medium was concentrated for about 100-fold using an Amicon Ultra-15 Centrifugal Filter Unites (Millipore, Billerica, MA) with a cut-off of 3000 daltons. The concentrated solute was supplemented with 10% FBS and antibiotics to make conditioned medium.
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