Maackia amurensis agglutinin

Endothelial Cell Growth Medium (cat. C-22010), penicillin/streptomycin solution, trypsin-EDTA solution, Lumi-Light PLUS Western Blotting Kit (including anti-mouse and anti-rabbit IgG-HRP secondary antibodies), protease and phosphatase inhibitor cocktails, extravidin-alkaline phosphatase (AP) conjugate, and 1-deoxymannojirimycin (DMJ) were purchased from Merck Group (Darmstadt, Germany). RPMI 1640 GlutaMAX™-I medium, DMEM medium, foetal bovine serum (FBS), Alamar Blue cell viability reagent, and MicroBCA Protein Assay kit were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The 2x concentrated Laemmli Buffer was obtained from Bio-Rad Laboratories (Hercules, CA, USA). Biotinylated lectins: Phaseolus vulgaris leukoagglutinin (PHA-L), Phaseolus vulgaris erythroagglutinin (PHA-E), Sambucus nigra agglutinin (SNA), Maackia amurensis agglutinin (MAA), Galanthus nivalis agglutinin (GNA), and Aleuria aurantia agglutinin (AAA) were purchased from Vector Laboratories Inc. (Burlingame, California, United States). ApogeeMix reference beads were purchased from Apogee Flow Systems (Northwood, UK). Polyvinyidene fluoride membranes (PVDF) were from Millipore (Burlington, MA, USA). All remaining chemicals were of analytical grade, commercially available.