Zli 9555

IHC for AR (ab9474, Abcam), FGFR1 (9740, CST), P-ERK (4370, CST), SOX2 (3579, CST), CHGA (ab45179, Abcam), SYP (ab52636, Abcam) and ARHGEF2 (ab155785, Abcam) was performed using PV-6000 system (ZSGB-BIO, China). Briefly, the slides were immersed in 1X Tris-EDTA (pH 9.0) buffer (Solarbio, China) and placed in a microwave oven for 10 min on high heat, and then adjusted to medium-low heat for 10 min to restore the antigen. 3% H₂O₂ was added to remove endogenous peroxidase in tissue samples. Cover the tissue on the slide with the primary antibody, place it in a humid box, and incubate overnight at 4 °C. After rewarming at room temperature for 30 min, horseradish peroxidase-linked secondary antibody (ZSGB-BIO, PV-6000, China) was added to the specimen and incubate the slides at room temperature for 30 min. After being stained with the DAB solution, the slides were immediately placed in water to stop dyeing, slides were subsequently counterstained with hematoxylin. The tissue is then dehydrated and preserved with neutral balsam (OriGene, ZLI- 9555, China).

IHC for AR (ab9474, Abcam), FGFR1 (9740, CST), P-ERK (4370, CST), SOX2 (3579, CST), CHGA (ab45179, Abcam), SYP (ab52636, Abcam) and ARHGEF2 (ab155785, Abcam) was performed using PV-6000 system (ZSGB-BIO, China). Briefly, the slides were immersed in 1X Tris-EDTA (pH 9.0) buffer (Solarbio, China) and placed in a microwave oven for 10 min on high heat, and then adjusted to medium-low heat for 10 min to restore the antigen. 3% H₂O₂ was added to remove endogenous peroxidase in tissue samples. Cover the tissue on the slide with the primary antibody, place it in a humid box, and incubate overnight at 4 °C. After rewarming at room temperature for 30 min, horseradish peroxidase-linked secondary antibody (ZSGB-BIO, PV-6000, China) was added to the specimen and incubate the slides at room temperature for 30 min. After being stained with the DAB solution, the slides were immediately placed in water to stop dyeing, slides were subsequently counterstained with hematoxylin. The tissue is then dehydrated and preserved with neutral balsam (OriGene, ZLI- 9555, China).

IHC for AR (ab9474, Abcam), FGFR1 (9740, CST), P-ERK (4370, CST), SOX2 (3579, CST), CHGA (ab45179, Abcam), SYP (ab52636, Abcam) and ARHGEF2 (ab155785, Abcam) was performed using PV-6000 system (ZSGB-BIO, China). Briefly, the slides were immersed in 1X Tris-EDTA (pH 9.0) buffer (Solarbio, China) and placed in a microwave oven for 10 min on high heat, and then adjusted to medium-low heat for 10 min to restore the antigen. 3% H₂O₂ was added to remove endogenous peroxidase in tissue samples. Cover the tissue on the slide with the primary antibody, place it in a humid box, and incubate overnight at 4 °C. After rewarming at room temperature for 30 min, horseradish peroxidase-linked secondary antibody (ZSGB-BIO, PV-6000, China) was added to the specimen and incubate the slides at room temperature for 30 min. After being stained with the DAB solution, the slides were immediately placed in water to stop dyeing, slides were subsequently counterstained with hematoxylin. The tissue is then dehydrated and preserved with neutral balsam (OriGene, ZLI- 9555, China).