HEK293T cells were obtained from ATCC. HeLa cells were a gifted by Sabrina Spencer. Beige fat cells were a gift from Shingo Kajimura. Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal calf serum (Invitrogen), penicillin, streptomycin (100units/mL), and 1 mM L-glutamine. Cells were treated at indicated concentrations with CCCP (SigmaAldrich), valinomycin (Tocris), Antimycin (SigmaAldrich) Oligomycin (Cayman Chemical), Actinonin (Cayman Chemical), FK506 (Cayman Chemical), 1-NA-PP1 (Cayman Chemical), Bafilomycin A1 (SigmaAldrich), and Ru360 (EMDMillipore). Antibodies were purchased from the following providers: anti-PDH, Abcam; anti-Tom20, Proteintech. Fluorescent labeled secondary antibodies were purchased from ThermoFisher.
Anti pdh
Manufactured by Abcam
Sourced in United States
Anti-PDH is a product that detects the pyruvate dehydrogenase (PDH) complex, a key enzyme involved in cellular energy metabolism. It is suitable for use in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Oligomycin, by Cayman Chemical (2 mentions)
Antimycin, by Merck Group (2 mentions)
Valinomycin, by Bio-Techne (2 mentions)
Ru360, by Merck Group (2 mentions)
Fluorescent labeled secondary antibody, by Thermo Fisher Scientific (2 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions)
Anti tom20, by Proteintech (2 mentions)
Fk506, by Cayman Chemical (2 mentions)
Actinonin, by Cayman Chemical (2 mentions)
1 na pp1, by Cayman Chemical (2 mentions)
Bafilomycin a1, by Merck Group (2 mentions)
Anti cleaved caspase 3, by Santa Cruz Biotechnology (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Terminal deoxynucleotidyl transferase mediated dutp nick end labeling tunel kit, by Roche (1 mentions)
Anti pdk1, by Abcam (1 mentions)
Anti p pdh, by Abcam (1 mentions)
Anti caspase 3, by Santa Cruz Biotechnology (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
3 protocols using anti pdh
1
Mitochondrial Function Modulation in Cell Lines
2
Investigating Metabolic Inhibitors in Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DIC was purchased from Selleckchem (Houston, TX, USA) and was dissolved in 2% dimethyl sulfoxide (DMSO). The glycolysis inhibitor DCA, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and Hoechst 33342 were purchased from Sigma (Shanghai, China). Anti-caspase-3, anti-cleaved-caspase-3, anti-poly (ADP-ribose) polymerase (PARP), anti-cleaved-PARP, and anti-β-actin antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-PDK1, anti-PDH, and anti-p-PDH antibodies were bought from Abcam Biotechnology (Cambridge, MA, USA). The terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) kit was purchased from Roche (Basel, Switzerland).
3
Mitochondrial Function Modulation in Cell Lines
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!