The protein lysate was subjected to SDS-PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane. Protein expression was analyzed by western blotting using primary antibodies against E-cadherin (Clone 36B5; NeoMarkers, Fremont, CA, USA), Vimentin (C-20; Santa Cruz Biotechnology, Santa Cruz, CA, USA), Slug (Santa Cruz Biotechnology), Twist (GeneTex), Annexin A2 (GeneTex), phosphorylated and total Akt (Cell Signaling Technology, Beverly, MA, USA), phosphorylated and total Erk (Cell Signaling Technology), or β-actin (Sigma-Aldrich), followed by incubation with horseradish peroxidase-conjugated secondary antibodies. Specific proteins were detected by chemiluminescence using ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences, Piscataway, NJ, USA).
Phosphorylated and total erk
Manufactured by Cell Signaling Technology
Sourced in United States, China
Phosphorylated and total Erk is a lab equipment product that measures the levels of both phosphorylated and total Erk (Extracellular signal-Regulated Kinase) proteins in cell lysates. Erk is a key signaling molecule involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Phosphorylated and total akt, by Cell Signaling Technology (2 mentions)
Ecl plus western blotting detection reagent, by GE Healthcare (1 mentions)
β actin, by Merck Group (1 mentions)
Twist, by GeneTex (1 mentions)
Sp7 osterix, by Abcam (1 mentions)
Hbmsc, by Cyagen (1 mentions)
Alexa fluor 555, by Beyotime (1 mentions)
Non phosphorylated active β catenin, by Cell Signaling Technology (1 mentions)
Runx2, by Beyotime (1 mentions)
Lipo 6000 transfection reagents, by Beyotime (1 mentions)
Serpinb2, by Abcam (1 mentions)
Hbmsc growth medium, by Cyagen (1 mentions)
Gapdh, by Beyotime (1 mentions)
2 protocols using phosphorylated and total erk
1
Western Blot Analysis of EMT Markers
2
Osteogenic Differentiation of hBMSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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hBMSCs were purchased from Cyagen Biosciences (Guangzhou, China). hBMSC growth medium were purchased from Cyagen Biosciences. Cells cultured in a atmosphere of 5% CO2 at 37 °C. Osteogenic induction medium was prepared according to previous methods [49 (link)].
SERPINB2 recombinant protein was purchased from Amyjet Scientific (Abnova, wuhan, china). Antibodies used for western blotting including GAPDH (1:1000, Beyotime, shanghai, china), RUNX2 (1:1000, Beyotime), COL1A1 (1:1000, Beyotime), SERPINB2 (1:1000, Abcam), SP7/Osterix (1:1000, Abcam, shanghai, china), non-phosphorylated (active) β-catenin (1:1000; Cell Signaling Technology), or total β-catenin (1: 1000; Beyotime), phosphorylated and total ERK (1:1000; Cell Signaling Technology), phosphorylated and total AKT (1:1000; Cell Signaling Technology). Second antibodies Alexa Fluor 555 was purchased from Beyotime. Lipo6000 Transfection Reagents were purchased from Beyotime (Shanghai, china).
The siRNAs were purchased from Genepharma (Hangzhou, china).
hSERPINB2 sense: GCAAAGAAUCAAGUUGCAATT
hSERPINB2 antisense: UUGCAACUUGAUUCUUUGCTT
hNC sense: UUCUCCGAACGUGUCACGUTT
hNC antisense: TTAAGAGGCUUGCACAGUGCA
mSERPINB2 sense: GGAGAGAAGUCUGCAAGAUTT
mSERPINB2 antisense: AUCUUGCAGACUUCUCUCCTT
mNC sense: GGCUCUGTTCUUGUCACGUAA
mNC antisense: UUTTGAGGCAAGCACAGUGCA
SERPINB2 recombinant protein was purchased from Amyjet Scientific (Abnova, wuhan, china). Antibodies used for western blotting including GAPDH (1:1000, Beyotime, shanghai, china), RUNX2 (1:1000, Beyotime), COL1A1 (1:1000, Beyotime), SERPINB2 (1:1000, Abcam), SP7/Osterix (1:1000, Abcam, shanghai, china), non-phosphorylated (active) β-catenin (1:1000; Cell Signaling Technology), or total β-catenin (1: 1000; Beyotime), phosphorylated and total ERK (1:1000; Cell Signaling Technology), phosphorylated and total AKT (1:1000; Cell Signaling Technology). Second antibodies Alexa Fluor 555 was purchased from Beyotime. Lipo6000 Transfection Reagents were purchased from Beyotime (Shanghai, china).
The siRNAs were purchased from Genepharma (Hangzhou, china).
hSERPINB2 sense: GCAAAGAAUCAAGUUGCAATT
hSERPINB2 antisense: UUGCAACUUGAUUCUUUGCTT
hNC sense: UUCUCCGAACGUGUCACGUTT
hNC antisense: TTAAGAGGCUUGCACAGUGCA
mSERPINB2 sense: GGAGAGAAGUCUGCAAGAUTT
mSERPINB2 antisense: AUCUUGCAGACUUCUCUCCTT
mNC sense: GGCUCUGTTCUUGUCACGUAA
mNC antisense: UUTTGAGGCAAGCACAGUGCA
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