Lytic buffer

Manufactured by Promega

Lytic buffer is a solution used for cell lysis, which is the process of breaking down the cell membrane and releasing the cellular contents. It is a core component in various molecular biology and biochemical applications, such as protein extraction, nucleic acid purification, and sample preparation for analysis.

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Lab products found in correlation

Lgbit, by Promega (2 mentions) Brefeldin a, by BioLegend (1 mentions) Pd153035, by Selleck Chemicals (1 mentions) Ml240, by Merck Group (1 mentions) Sb203580, by Merck Group (1 mentions) Colchicine, by Fujifilm (1 mentions) Thapsigargin, by Fujifilm (1 mentions) Vincristine, by Fujifilm (1 mentions)

Spelling variants of this product

Nano-Glo HiBiT Lytic Buffer (6 citations)

2 protocols using lytic buffer

High-Throughput Screening of LOPAC Library

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At first, 4×10⁴ PC9-KI cells were seeded on 96 well plates. Then, after 24 h, 1μM of compounds in the LOPAC chemical compound library (Sigma Aldrich, MO, USA) were added to each well. Following 24h, the medium was discarded, and a detection mix (PBS 12.5 μL, lytic buffer 12.5 μL, substrate (Promega) 0.25 μL, and LgBiT (Promega) 0.125μL) was added to each well. The plates were incubated at room temperature for 10 min in the dark, and the luminescence was measured using ARVO X3.

Uchida Y., Matsushima T., Kurimoto R., Chiba T., Inutani Y, & Asahara H. (2021). Identification of chemical compounds regulating PD-L1 by introducing HiBiT-tagged cells. FEBS letters, 595(5), 563-576.

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Cytotoxicity Screening of Drug Candidates

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First, 4×10⁴ of PC9-KI cells were seeded on 96 well plates. After 24 h, 0.1 % of DMSO or 10⁻⁵μM, 10⁻⁴μM, 10⁻³μM, 10⁻²μM, 10⁻¹μM, 1 μM, or 10 μM of SB203580 (Sigma Aldrich), brefeldin A (BioLegend, CA, USA), PD153035 (Selleck, TX, USA), colchicine (WAKO), vincristine (Cayman, Michigan, USA), thapsigargin (WAKO, Osaka, Japan), and ML-240 (Sigma Aldrich, MO, USA) were added. After 24 h, the medium was discarded, and detection mix (PBS 12.5 μL, lytic buffer (Promega) 12.5 μL, substrate (Promega) 0.25 μL, and LgBiT (Promega) 0.125 μL) was added to each well.

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