Diaminobenzidine tetrahydrochloride solution kit hk153 5k

Formalin-fixed, paraffin-embedded, 4-µm tumor sections were dewaxed in xylene, rehydrated with graded alcohol concentrations, and placed in an endogenous peroxide blocking buffer for 15 minutes^{17 (link)}. Sections were washed in water, antigen-retrieved, and placed in citrate buffer^{17 (link)}. Nonreactive staining was blocked by treating the sections with 1% horse serum in Tris-buffered saline (pH 6.0) for 3 minutes^{17 (link)}. Ki-67 antibody (clone MIB1, IS626, 1:200; DAKO, Denmark) was then applied and the binding of antibodies was detected using the avidin-biotin-peroxidase complex (Universal Elite ABC Kit; Vectastain, Burlingame, CA, USA) for 10 minutes. Diaminobenzidine tetrahydrochloride solution (Kit HK153–5K; Biogenex, San Ramon, CA, USA) was used as a chromogen^{17 (link)}. The tumor specimens were stained with hematoxylin and eosin (H&E) for the examination of the basic histomorphological features^{17 (link)}.

Formalin-fixed, paraffin-embedded, 4-μm tumor sections were dewaxed in xylene, rehydrated with graded alcohol concentrations, and placed in an endogenous peroxide blocking buffer for 15 minutes. Sections were washed in water, antigen-retrieved, and then placed in citrate buffer. Nonreactive staining was blocked by treating the sections with 1% horse serum in Tris-buffered saline (pH 6.0) for 3 minutes. Then, anti-KIT (1:800, A4502, DAKO, Carpinteria, CA, USA) and anti-phosphatase and tensin homolog (PTEN, 1:100, ab32199; Abcam, Cambridge, MA, USA) antibodies were then applied and the antibody binding was detected using an avidin-biotin peroxidase complex (Universal Elite ABC kit, Vectastain, Burlingame, CA, USA) for 10 minutes. Diaminobenzidine tetrahydrochloride solution (Kit HK153-5K; Biogenex, San Ramon, CA, USA) was then used as a chromogen. The tumors specimens were stained with hematoxylin and eosin (H&E) to examine the basic histomorphological features.