Agilent 1100 hplc dad

An Agilent 1100 HPLC-DAD was used for the chromatographic analysis (Agilent Technologies, California, USA). On a Luna Omega Polar C18 column (5 μm, 100 Å, 4.6 mm × 250 mm) (Phenomenex Inc., Torrance, CA, USA), the separation runs were carried out at 40 °C. A mixture of aqueous acetic acid (0.1% v/v) and methanol (35:65) was used as a mobile phase. The injection volume was 20 μL, and the flow rate was 0.8 mL/min with isocratic elution. The detection UV wavelength was set at 254 nm.
The electrospun shellac fibers loaded with KP extract were successfully powdered. A 100 mg sample powder was precisely weighed into a 1.0 mL dry, clean volumetric flask. To aid in full solubility, the mobile phase was added and ultrasonically mixed. The same mobile phase was used to adjust the volume, and the analysis was performed using the 100 mg/mL sample solution. The validated method was then used to simultaneously measure the amounts of DMF, TMF, and PMF in various electrospun shellac fibers loaded with KP extract.
In the case of sample solutions obtained from an in vitro dissolution study of KP extract-loaded electrospun shellac fibers, each sample from the dissolution vessels was directly injected into the HPLC-DAD system, and the concentrations of methoxyflavones in each sample were calculated using their corresponding calibration curves.

The Agilent 1100 HPLC–DAD (Agilent Technologies, CA, USA) was used for all analyses. All chromatographic conditions were carried out with isocratic elution utilizing a Luna Omega Polar C18 column (5 μm, 100 Å, 4.6 mm × 250 mm) (Phenomenex Inc., Torrance, CA, USA), a flow rate of 1 mL/min and a detection wavelength of 254 nm, with some changes as detailed in Table 4. By comparing the unknown substance’s retention time and UV spectrum to the standard, rhein was found.