Mice injected intravenously with ssAAV8-HLP-Luciferase at the indicated doses were monitored non-invasively for the whole-body distribution of the transgene on day 10. Mice were injected intraperitoneally with 2 mg/mouse XenoLight D-Luciferin (Perkin Elmer, Waltham, USA), anesthetised with isofluorane and imaged 15 min later using the IVIS Spectrum pre-clinical imaging system (Perkin Elmer, Waltham, USA). The bioluminescence was quantified from ROIs with deducted background signal using the Living Image 3.2 In Vivo Imaging Software (Perkin Elmer).
Living image 3.2 in vivo imaging software
Manufactured by PerkinElmer
Sourced in United States
The Living Image 3.2 In Vivo Imaging Software is a tool for visualizing and analyzing data from in vivo imaging experiments. It provides image acquisition, processing, and analysis capabilities.
Automatically generated - may contain errors
2 protocols using living image 3.2 in vivo imaging software
1
Non-invasive Bioluminescence Imaging of AAV8 Transduction
2
In Vivo Monitoring of Lung Tumor Growth
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Bioluminescent SW756 cells (1 x 10 6 in 200 μl), subjected to different treatments, were injected into the lateral tail vein of 5-6-week old female NOD SCID (NOD.CB17-Prkdcscid/NcrCrl) mice (Charles River, Oxford, UK), using 29-gauge insulin needles. Growth of lung tumours was monitored noninvasively using the Living Image 3.2 In Vivo Imaging Software (PerkinElmer, Waltham, MA, USA). Initial measurements were made 30 min after tail vein injection, to confirm equal cell access to the lungs, followed by serial whole-body imaging at weekly intervals. All mice were maintained in conventional cages within a specific pathogen-free animal facility. They were treated in strict accordance with guidelines from the Cambridge University Licence Review Committee and the UK Home Office. The data obtained using the In Vivo Imaging Software were confirmed by two independent methods, namely histology and qPCR measurement of human DNA levels in the mouse lung tissue at the end of the experiment. Details are given in supplementary material, Materials and methods.
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