All chemicals and reagents were purchased from MilliporeSigma (Burlington, MA, USA) and used as received without further purification unless otherwise noted. Aqueous solutions were made in nanopure deionized water (H2O) from Milli-Q water (MilliporeSigma). Ammonium acetate (AA) was purchased from Fisher Scientific (Fair Lawn, NJ). Bradford protein assay reagent was purchased from Bio-Rad (Hercules, CA, USA). A 10% gel (10 or 15 comb well, 10.0 cm × 10.0 cm, 1.0 mm thick) for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) was homemade. Thermo Scientific Cimarec+ stirring hot plates were purchased from ThermoFisher Scientific. Amicon 0.5 mL cellulose ultra centrifugal filters with a molecular weight cutoff (MWCO) of 10 kDa were purchased from MilliporeSigma. The recombinant SARS-CoV-2 spike regional-binding domain protein expressed in the HEK 293 cell line was purchased from Sino Biological Inc. (cat. 40592-VNAH).
Hek 293 cell line
Manufactured by Sino Biological
Sourced in United States
The HEK 293 cell line is a widely used immortalized cell line derived from human embryonic kidney cells. It is a common tool in cell biology research and biotechnology applications.
Automatically generated - may contain errors
2 protocols using hek 293 cell line
1
SARS-CoV-2 Spike Protein Purification
2
Overexpression of GR Modulates miR-218 in HEK-293 Cells
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We used HEK-293 cell line to transfect GR overexpression clone (SinoBiologicals Inc., Wayne, PA, USA). In parallel, control group was prepared by transfecting the cell line with scramble plasmid. Forty-eight hours post-transfection, cells were harvested, and RNA was extracted as mentioned above. The extracted RNA was used in miRNA-specific cDNA preparation and subsequently used in qPCR with primers to determine miR-218 expression. The expression was normalized by U6 gene as reference. The fold-change was determined as mentioned above.
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