Antibodies against WHSC1 (1:1000, 65127S; Cell Signaling Technology, Beverly, MA, USA), mTOR (1:1000, 2983T; Cell Signaling Technology, Beverly, MA, USA), P70S6K (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA), S6 (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA), p-mTOR (1:1000, 5536T; Cell Signaling Technology, Beverly, MA, USA), p-P70S6K (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA), p-S6 (1:1000, 4858T; Cell Signaling Technology, Beverly, MA, USA), OXCT1 (1:1000, 12,175-1-AP; Proteintech, Wuhan, Hubei, China), RUVBL2 (1:1000, 10,195-1-AP; Proteintech, Wuhan, Hubei, China), TXNRD1 (1:1000, 11,117-1-AP; Proteintech, Wuhan, Hubei, China), HSD17B4 (1:1000, 15,116-1-AP; Proteintech, Wuhan, Hubei, China), PKM (1:1000, 10,078-2-AP; Proteintech, Wuhan, Hubei, China), EIF3A (1:1000, 26,178-1-AP; Proteintech, Wuhan, Hubei, China), TPM4 (1:1000, 13,741-1-AP; Proteintech, Wuhan, Hubei, China), and P4HB (1:1000, ab2792; Abcam, Cambridge, UK) were used in this study. Western blotting was performed as previously described.17 (link)
Ruvbl2
Manufactured by Proteintech
Sourced in China
RUVBL2 is a protein that is part of the RuvB-like protein family. It functions as a DNA helicase involved in various cellular processes, including transcription regulation, DNA repair, and chromatin remodeling.
Automatically generated - may contain errors
Lab products found in correlation
P p70s6k, by Cell Signaling Technology (1 mentions)
Hsd17b4, by Proteintech (1 mentions)
Eif3a, by Proteintech (1 mentions)
Txnrd1, by Proteintech (1 mentions)
P mtor, by Cell Signaling Technology (1 mentions)
P70s6k, by Cell Signaling Technology (1 mentions)
Oxct1, by Proteintech (1 mentions)
Ruvbl1, by Proteintech (1 mentions)
Ecl plus, by GE Healthcare (1 mentions)
2 protocols using ruvbl2
1
Western Blotting of Cellular Proteins
2
Western Blot Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Equal amounts of cell lysate were mixed with SDS sample buffer (4×) and heated at 90°C for 5 min. After centrifugation at 3000×g for 30 s, sample (25 μg) was loaded on a 4–12% or 4–20% SDS-PAGE. Proteins were transferred to nitrocellulose membranes and stained with Ponceau S. The nitrocellulose membranes were blocked with 5% milk/TBST for 5 min to remove Ponceau S and for additional 30 min. Primary antibodies were prepared in 3% milk/TBST and incubated for 1 hr at room temperature on a shaker. After removing the primary antibody, membranes were washed three times with 3% milk/TBST for 5 min each. Primary antibodies were used including rabbit polyclonal antibodies against human RUVBL1 and RUVBL2 (Proteintech), anti-β-tubulin and anti-β-actin. Secondary antibodies were incubated for 1 hr at room temperature on a shaker. Membranes were washed three times with TBST with 5 min each time. ECL Plus (GE healthcare) was used to detect signal.
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