Psychrobacter sp. ANT 206 (GenBank accession numbers MK968312), isolated from a sample of Antarctic sea-ice (68° 30’ E, 65° 00’ S), was cultured in 2216E sea water medium (5 g of peptone (Sinopharm, Beijing, China) and 1 g of yeast extract powder (Sinopharm, Beijing, China) per 1 L of solution, pH 7.5) at 12 °C with shaking at 200 rpm and then frozen in glycerol at −80 °C for long term storage. Psychrobacter sp. ANT206 was used as a source of chromosomal DNA. All strains were stored in 16% glycerol at −20 °C, BL21/pET-28a(+) and E. coli BL21 were cultured in Luria Bertani (LB) medium (10 g of tryptone (Sinopharm, Beijing, China), 5 g of yeast extract powder, and 10 g of NaCl (Sinopharm, Beijing, China) per 1 L of solution, pH 7.0) at 37 °C.
Yeast extract powder
Yeast extract powder is a food-grade product derived from the deactivation and autolysis of Saccharomyces cerevisiae yeast cells. It contains a variety of amino acids, vitamins, and minerals that are essential for microbial growth and development. The powder form provides a concentrated source of these nutrients.
Lab products found in correlation
2 protocols using yeast extract powder
Antarctic Psychrobacter sp. ANT206 Cultivation
Psychrobacter sp. ANT 206 (GenBank accession numbers MK968312), isolated from a sample of Antarctic sea-ice (68° 30’ E, 65° 00’ S), was cultured in 2216E sea water medium (5 g of peptone (Sinopharm, Beijing, China) and 1 g of yeast extract powder (Sinopharm, Beijing, China) per 1 L of solution, pH 7.5) at 12 °C with shaking at 200 rpm and then frozen in glycerol at −80 °C for long term storage. Psychrobacter sp. ANT206 was used as a source of chromosomal DNA. All strains were stored in 16% glycerol at −20 °C, BL21/pET-28a(+) and E. coli BL21 were cultured in Luria Bertani (LB) medium (10 g of tryptone (Sinopharm, Beijing, China), 5 g of yeast extract powder, and 10 g of NaCl (Sinopharm, Beijing, China) per 1 L of solution, pH 7.0) at 37 °C.
Cultivation of P. sajor-caju Mushroom
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