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Ndufv2

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NDUFV2 is a subunit of the NADH dehydrogenase (ubiquinone) 1 beta complex, which is part of the mitochondrial respiratory chain. It is involved in the first step of the electron transport chain, the oxidation of NADH to NAD+.

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Lab products found in correlation

Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) β actin, by Proteintech (1 mentions) Nlrp3, by Proteintech (1 mentions) Vinculin, by Merck Group (1 mentions) Ndufb8, by Thermo Fisher Scientific (1 mentions) Cpt1a, by Proteintech (1 mentions) Total oxphos rodent wb antibody cocktail, by Abcam (1 mentions) Gapdh, by GeneTex (1 mentions) Vimentin antibody, by Proteintech (1 mentions) Hsp60 antibody, by Stressgen (1 mentions) Phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Histone h3 antibody, by Beyotime (1 mentions) Ndufv1, by Proteintech (1 mentions) Ndufs3, by Proteintech (1 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Ampkα, by Cell Signaling Technology (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions)

3 protocols using ndufv2

1

Western Blot Analysis of Immune Signaling

Cited 1 time
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Western blotting analysis was performed as previously described (Wang et al. 2017 (link)). Briefly, total proteins from cultured cells were obtained, separated by SDS–PAGE electrophoresis and transferred onto nitrocellulose membranes. Primary antibodies against IL-1β (1:1000, CST), NLRP3 (1:500, Proteintech), NF-κB (1:1000, CST), p-NF-κB (1:1000, CST), Atp5a1 (1:2000, Proteintech), Ndufv2 (1:5000, Proteintech), β-actin (1:10,000, Proteintech) and appropriate secondary antibodies were used. The optical density of bands was analyzed by ImageJ software. The expression levels of the examined proteins were normalized to those of β-actin.
Li X., Zhou X., Liu X., Li X., Jiang X., Shi B, & Wang S. (2022). Spermidine protects against acute kidney injury by modulating macrophage NLRP3 inflammasome activation and mitochondrial respiration in an eIF5A hypusination-related pathway. Molecular Medicine, 28, 103.
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2

Adipose Mitochondrial OXPHOS Protein Analysis

Cited 1 time
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Immunoblotting of adipose mitochondria for the different OXPHOS proteins was done as previously described5 (link). Primary antibodies used were Total OXPHOS Rodent WB Antibody Cocktail (1/5,000 dilution, no. ab110413, Abcam), SDHA (1/5,000 dilution, no. 459200, Thermo Fisher Scientific), NDUFB8 (1/5,000 dilution, no. 459210, Thermo Fisher Scientific), LCAD (1/5000 dilution, no. ab129711, Abcam), CPT1a (1/2000 dilution, no. 15184–1-AP, Proteintech), UCP1 (1/5,000 dilution, no. ab10983, Abcam), NDUFV2 (1/5,000 dilution, no. 15301–1-AP, Proteintech), MYC (1/2,000 dilution, no. 05–724, Millipore), GAPDH (1/5,000 dilution, no. GTX100118, GeneTex) and Vinculin (1/5,000 dilution, no. V9131, Sigma). Image contrast was uniformly reduced to enhance visibility. Band densitometry was quantified using ImageJ Gel Plugin (National Institutes of Health (NIH)).
Krishnan K.C., Vergnes L., Acín-Pérez R., Stiles L., Shum M., Ma L., Mouisel E., Pan C., Moore T.M., Péterfy M., Romanoski C.E., Reue K., Björkegren J.L., Laakso M., Liesa M, & Lusis A.J. (2021). Sex-specific genetic regulation of adipose mitochondria and metabolic syndrome by Ndufv2. Nature metabolism, 3(11), 1552-1568.
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3

Comprehensive Protein Analysis Protocol

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Cells were lysed in RIPA buffer supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail (Thermo, Waltham, MA). Equal amount of proteins were separated on the 12% SDS-PAGE gel and transferred onto a PVDF transfer membrane. Western blot analysis followed a standard procedure. P70 S6 Kinase, phospho-p70 S6 Kinase (Thr389) β-catenin and FASN antibodies were obtained from Sigma (St Louis, MO). AMPKα, phospho-AMPKα (Thr172), Raptor, phosphor-Raptor (Ser792), 4EBP1, phospho-4E-BP1 (Thr37/46), ACC, N-cadherin, SRC, LEF1 and β-actin were obtained from Cell Signaling Technology (Danvers, MA). NDUFV1, NDUFV2, NDUFS3, RPS9 and RPL18A were obtained from Proteintech (Chicago, IL). NDUFA5 antibody was obtained from Pierce (Rockford, IL). HSP60 antibody was obtained from Stressgen (Victoria, BC). Vimentin antibody was obtained from Proteintech (Chicago, IL). Histone H3 antibody was obtained from Beyotime(shanghai, China).
Tang H., Li J., Liu X., Wang G., Luo M, & Deng H. (2016). Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway. Scientific Reports, 6, 28388.
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