Mcherry lc3b

Manufactured by Addgene

MCherry-LC3B is a plasmid construct that expresses the mCherry fluorescent protein fused to the LC3B protein. LC3B is a key component of the autophagy pathway in cells. The mCherry fluorescent tag allows for the visualization and tracking of LC3B and the autophagic process in live cells.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Lamp1 mcherry, by Addgene (1 mentions) Mrfp gfp lc3b, by Addgene (1 mentions) Peyfp c1 drp1, by Addgene (1 mentions) Lamp1 mgfp, by Addgene (1 mentions) Mfn2 yfp, by Addgene (1 mentions) Egfp lc3, by Addgene (1 mentions)

2 protocols using mcherry lc3b

Plasmid Transfection and Autophagy Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cDNA plasmids mCherry-LC3B (Cat# 40827), mRFP-GFP-LC3B (Cat# 21074), and Lamp1-mCherry (Cat# 45147) were purchased from Addgene. All plasmids were validated by sequencing. For transient transfections, the cells were plated at 60%–70% confluence. After 12 h, the cDNAs were transfected with Lipofectamine 2000 (Thermo Fisher Scientific, Cat# 11668019) using the forward transfection protocol provided by the manufacturer. The siRNA target sequences are as follows: non-targeting control siRNA, 5′-UUCUCCGAACGUGUCACGUTT-3′; human ATG5_siRNA, 5′-GCCUGUAUGUACUGCUUUA-3′; mouse Atg5_siRNA, 5′-CUCUCUAUCAGGAUGAUTT-3′; all the siRNAs have been validated by western blot showing > 80% knockdown of the target.

Fu Y., Chen N., Wang Z., Luo S., Ding Y, & Lu B. (2021). Degradation of lipid droplets by chimeric autophagy-tethering compounds. Cell Research, 31(9), 965-979.

+ Open protocol

+ Expand

Plasmid Constructs for CHCHD10 and TDP43

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

All complementary DNAs (cDNAs) for human CHCHD10^WT and variants were synthesized and inserted in the pcDNA3 vector containing a FLAG, HA, or Myc tag by Genescripts. mTagRFP-T-Mito-7 (#58023)^{64 (link)}, TDP43tdTOMATO-HA (#28205)⁶⁵, EGFP-LC3 (#21073)⁶⁶, mCherry-LC3B (#40827)^{67 (link)}, pEYFP-C1-DRP1 (#45160)^{68 (link)}, MFN2-YFP (#28010)^{69 (link)}, pEYFP-N1-PINK1 (#101874)⁷⁰, and LAMP1-mGFP (#34831)^{71 (link)} plasmids were obtained from Addgene. FLAG-MFN2^WT, FLAG-MFN2^S378A, and FLAG-MFN2^S378D were described previously^{51 (link)}. TARDBP^WT, TARDBP^G298S, TARDBP^A315T, and TARDBP^A382T plasmids were gifts from Xinglong Wang. Mito-QC (pBabe.hygro-mcherry-GFP fis 101-152) was provided by Ian Ganley.

Baek M., Choe Y.J., Bannwarth S., Kim J., Maitra S., Dorn GW I.I., Taylor J.P., Paquis-Flucklinger V, & Kim N.C. (2021). TDP-43 and PINK1 mediate CHCHD10S59L mutation–induced defects in Drosophila and in vitro. Nature Communications, 12, 1924.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!