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Resolvin e1 rve1

Manufactured by MyBioSource
Sourced in United States

Resolvin E1 (RvE1) is a lipid mediator that is derived from the omega-3 fatty acid eicosapentaenoic acid (EPA). RvE1 plays a role in the resolution of inflammation and promotes the restoration of homeostasis.

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3 protocols using resolvin e1 rve1

1

Measuring Inflammatory and Resolving Mediators

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Protein levels of interleukin (IL)-6, IL-1β, Monocyte Chemoattractant Protein (MCP)-1, and Tumor Necrosis Factor (TNF)-α (Biolegend enzyme-linked immunosorbent assay kits, San Diego, CA, USA), and Resolvin E1 (RVE1) and Resolvin D1 (RVD1) (MyBiosource, San Diego, CA, USA), were measured in plasma. Enzyme-linked immunosorbent assays (ELISA) were performed according to manufacturer’s instructions. Exact kits are enumerated in Table S3.
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2

Quantifying Inflammatory Lipid Mediators

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Serum concentrations of leukotriene B4 (LTB4), leukotriene B5 (LTB5) (Cusabio, Houston, TX, USA), prostaglandin E2 (PGE2), prostaglandin E3 (PGE3) (MyBioSource, MyBioSource Inc., San Diego, CA, USA), resolvin E1 (RvE1) (MyBioSource, MyBioSource Inc., CA, USA) were measured by commercially available enzyme-linked immunosorbent assay (ELISA) kits on compact absorbance reader for 96-well microplates (BioRad PR 3100 TSC, Bio-Rad Laboratories, CA, USA).
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3

Quantification of Inflammatory Cytokines and Resolvins

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The supernatants from cell culture medium (ex-vivo) of non-elicited and elicited peritoneal Mφs were analyzed for three pro-inflammatory cytokines: IL-1β, IL-6, and TNF-α (R&D Systems, Inc., Minneapolis, MN, USA), and three resolvins: RvD1 (Cayman Chemical., Ann Arbor, Michigan, USA), Resolvin E1 (RvE1) (MyBioSource, Inc., San Diego, CA, USA), and Lipoxin A4 (LxA4) (Neogen Corporation, Lexington, Kentucky, USA) by ELISA, and performed according to manufacturer’s instructions.
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