Rapamycin (#9904), wortmannin (#9951) and rabbit monoclonal antibodies against Akt (#4691), p-Akt (Thr308; #2965), p-Akt (Ser473; #9271), mTOR (#2983), p-mTOR (Ser2448; #5536), p70s6k (#2708), p-p70s6k (Thr389; #9234) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; #2118) were obtained from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-ATP5B monoclonal antibody (sc-135903) was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit anti-ATP5J polyclonal antibody (ab65841) was purchased from Abcam (Cambridge, MA, USA). HRP-conjugated goat secondary antibodies (AP307P and AP308P) were obtained from Millipore (Billerica, MA, USA). Alexa Fluor® 488-conjugated secondary antibodies (A27034 and A28175) were obtained from Invitrogen (Carlsbad, CA, USA). ECL reagent was purchased from GE Healthcare (Pittsburgh, PA, USA), and the BCA Protein assay kit was obtained from Pierce Chemical Co. (Rockford, IL, USA).
P akt thr308 2965
Manufactured by Cell Signaling Technology
Sourced in United States
P-Akt (Thr308; #2965) is a lab equipment product offered by Cell Signaling Technology. It is an antibody that specifically detects phosphorylation of Akt at Thr308.
Automatically generated - may contain errors
Lab products found in correlation
P akt ser473, by Cell Signaling Technology (1 mentions)
Ecl reagent, by GE Healthcare (1 mentions)
Rapamycin, by Cell Signaling Technology (1 mentions)
Wortmannin, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Rabbit monoclonal antibodies, by Cell Signaling Technology (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Multi gauge version 2, by Fujifilm (1 mentions)
Las 3000 luminescent image analyzer, by Fujifilm (1 mentions)
Ab33985, by Abcam (1 mentions)
Protein assay reagent, by Bio-Rad (1 mentions)
2 protocols using p akt thr308 2965
1
Signaling Pathway Antibody Validation
2
Protein Quantification and Western Blotting in Lung Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein concentration in homogenized lung samples was determined using the Bio-Rad Protein Assay reagent (Bio-Rad, Hercules, CA, USA). Western blotting was performed according to a previously described procedure [9 (link)] using antibodies against the following proteins: acetyl coenzyme (Co)A (#3676), phosphorylated (p)-acetyl CoA (#3661), LC3 (#2275), autophagy protein (ATG)5 (#2630), phosphorylated (p)-p70 S6 KinaseThr389(#9205), 4EBP1 (#9644S), and p-AktThr308 (#2965) (all from Cell Signaling Technology, Danvers, MA, USA); succinate dehydrogenase complex, subunit A (SDHA) (ab14715) and cytochrome c oxidase subunit (COX)-IV (ab33985) (both from Abcam); cytochrome c (A-8), Rieske (A-5), B-cell lymphoma (Bcl)-2 (C-2), Bcl-2-associated death promoter (Bad) (C-7), Bcl-2-associated X protein (Bax) (B-9), eIF4E (P-2), fibroblast growth factor (FGF)-2 (147), proliferating cell nuclear antigen (PCNA) (PC10), N-cadherin, and actin (I-19) (all from Santa Cruz Biotechnology); and glyceraldehyde-3-phosphate dehydrogenase (LF-PA0212; AbFrontier, Seoul, Korea). Protein bands were detected using the LAS-3000 luminescent image analyzer (Fujifilm, Tokyo, Japan) and quantified using Multi Gauge version 2.02 software (Fujifilm).
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