AAV9 was used to construct the shRNAs targeting the C5aR2, Cyp2e1, and Sirt1 genes. AAV9-Sirt1 was developed for overexpression of Sirt1 (GeneChem, Shanghai, China). Recombinant virus (1.5 × 1011 v.g) was diluted in 0.2 mL of a saline solution and injected into the tail vein of mice. One week post injection, the mice were subjected to a short-term binge feeding.
Sirt1
Manufactured by Genechem
Sourced in China
SIRT1 is a lab equipment product that serves as a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase enzyme. It plays a role in the regulation of cellular processes, including metabolism, stress response, and longevity.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using sirt1
1
AAV9-mediated Gene Manipulation in Mice
2
SIRT1, p53, GPX4 Knockdown in HL7702 Cells
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SIRT1, p53, GPX4, and negative control (NC) short interfering (siRNAs) were obtained from GeneChem (China). HL7702 cells were transfected with siRNAs using Lipofectamine 3000 (Invitrogen, United States) by following the manufacturer’s instructions. After 6 h, the culture medium was replaced with fresh complete medium and the cells were cultured for 48 h.
3
Elucidating Molecular Pathways in I/R Injury
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SIRT1, Akt, FOXO3a, and PGC-1α-specific short interfering RNA (siRNA) molecules were chemically synthesized by Shanghai Genechem Company. Transfection was performed by utilizing Lipofectamine RNAiMAX reagent as per the manufacturer's protocol (Life Technologies, CA, USA). After 48 h transfection, cells were treated with sAT for 24 h and then subjected to I/R for another 9 h.
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