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Minion sequencer platform

Manufactured by Oxford Nanopore
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Sourced in United Kingdom

The MinION is a portable, real-time DNA/RNA sequencing device developed by Oxford Nanopore. It is a nanopore-based sequencing platform that provides direct, electronic detection of nucleic acid molecules.

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Lab products found in correlation

Rapid sequencing kit, by Oxford Nanopore (2 mentions) Nextera dna flex library prep kit, by Illumina (2 mentions) Novaseq 6000 platform, by Illumina (2 mentions) Genomic tip 20 g column, by Qiagen (1 mentions)

Close spelling variants of this product

MinION sequencer MinION platform MinION

2 protocols using minion sequencer platform

1

Genomic DNA Isolation and Sequencing of Bacteroides ovatus

Check if the same lab product or an alternative is used in the 5 most similar protocols
B. ovatus (MDA-HVS BO001) genomic DNA was isolated and purified using a Qiagen Genomic-tip 20/G column, according to the manufacturer’s instructions. For short-read Illumina sequencing, libraries were constructed with a Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s protocol. All libraries were quantified with a TapeStation and pooled in equal molar ratios. The final libraries were sequenced with the NovaSeq 6000 platform (Illumina) to produce 2×150 bp paired-end reads, resulting in ~5 Gb per sample. For long-read Nanopore sequencing, 500 ng of genomic DNA was used for library preparation using the Rapid Sequencing Kit (SQK-RAD004, Oxford Nanopore Technologies). Libraries were loaded into a FLO-MIN106 flow-cell for a 24-h sequencing run on a MinION sequencer platform (Oxford Nanopore Technologies, Oxford, UK). Data acquisition and real-time base calling were carried out by the MinKNOW software version 3.6.5. The fastq files were generated from basecalled sequencing fast5 reads.
Hayase E., Hayase T., Mukherjee A., Stinson S.C., Jamal M.A., Ortega M.R., Sanchez C.A., Ahmed S.S., Karmouch J.L., Chang C.C., Flores I.I., McDaniel L.K., Brown A.N., El-Himri R.K., Chapa V.A., Tan L., Tran B.Q., Pham D., Halsey T.M., Jin Y., Tsai W.B., Prasad R., Glover I.K., Ajami N.J., Wargo J.A., Shelburne S., Okhuysen P.C., Liu C., Fowler S.W., Conner M.E., Peterson C.B., Rondon G., Molldrem J.J., Champlin R.E., Shpall E.J., Lorenzi P.L., Mehta R.S., Martens E.C., Alousi A.M, & Jenq R.R. (2023). Bacteroides ovatus alleviates dysbiotic microbiota-induced intestinal graft-versus-host disease. Research Square.
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2

Dual-Platform DNA Sequencing Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
BT (MDA-JAX BT001) genomic DNA was isolated and purified using a
Qiagen Genomic-tip 20/G column, according to the manufacturer’s
instructions. For short-read Illumina sequencing, libraries were constructed
with a Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA, USA),
according to the manufacturer’s protocol. All libraries were
quantified with a TapeStation and pooled in equal molar ratios. The final
libraries were sequenced with the NovaSeq 6000 platform (Illumina) to
produce 2×150 bp paired-end reads, resulting in ~5 Gb per
sample. For long-read Nanopore sequencing, 500 ng of genomic DNA was used
for library preparation using the Rapid Sequencing Kit (SQK-RAD004, Oxford
Nanopore Technologies). Libraries were loaded into a FLO-MIN106 flow-cell
for 24h sequencing run on a MinION sequencer platform (Oxford Nanopore
Technologies, Oxford, UK). Data acquisition and real-time base calling were
carried out by the MinKNOW software version 3.6.5. The fastq files were
generated from basecalled sequencing fast5 reads.
Hayase E., Hayase T., Jamal M.A., Miyama T., Chang C.C., Ortega M.R., Ahmed S.S., Karmouch J.L., Sanchez C.A., Brown A.N., El-Himri R.K., Flores I.I., McDaniel L.K., Pham D., Halsey T., Frenk A.C., Chapa V.A., Heckel B.E., Jin Y., Tsai W.B., Prasad R., Tan L., Veillon L., Ajami N.J., Wargo J.A., Galloway-Peña J., Shelburne S., Chemaly R.F., Davey L., Glowacki R.W., Liu C., Rondon G., Alousi A.M., Molldrem J.J., Champlin R.E., Shpall E.J., Valdivia R.H., Martens E.C., Lorenzi P.L, & Jenq R.R. (2022). Mucus-degrading Bacteroides link carbapenems to aggravated graft-versushost disease. Cell, 185(20), 3705-3719.e14.
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