G33 500

Manufactured by Thermo Fisher Scientific

The G33-500 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform a core function within a laboratory setting. The detailed specifications and intended use of this product are not available in a concise, unbiased, and factual format.

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Lab products found in correlation

Nicotine free base, by Merck Group (1 mentions)

2 protocols using g33 500

Nicotine Vapor Exposure in Mice

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Mice were placed in chambers for vaporized delivery of 120 mg/ml (-)-nicotine free base (Sigma N3876) in a 30/70 (v/v) PG (Sigma P4347)/VG (Fisher G33-500) solution or PG/VG control solution. Either 120 mg/ml Nic or PG/VG solution was filled into e-vape tanks (Baby Beast Brother, Smok) that were then screwed into the vapor generator (95 W, Model SVS200, La Jolla Alcohol Research, Inc) that triggers the heating of the vape solution into vapor. The vape generator was connected to the e-vape controller (Model SSV-1, La Jolla Alcohol Research, Inc) that controls duration and frequency of vape delivery. The air-tight chambers are connected to a vacuum system that constantly pulls room air through the chambers at ∼1 l/min and ensures that each triggered vape is pulled into the chamber. Each 3-s vape puff takes ∼1 min to clear the chamber. Vape exposure sessions start between 9 and 10 A.M. during the animals’ light cycle, but vapor exposure was administered in the dark with lights off in the room. Repeated exposure was performed on consecutive days over the same approximate time period. After vape exposure, mice were returned to their home cage and regular housing facility.

Zhu M., Echeveste Sanchez M., Douglass E.A., Jahad J.V., Hanback T.D., Guhr Lee T.N., Esther CR J.r., Cole M., Roberts A.J, & Herman M.A. (2021). Electronic Nicotine Vapor Exposure Produces Differential Changes in Central Amygdala Neuronal Activity, Thermoregulation and Locomotor Behavior in Male Mice. eNeuro, 8(4), ENEURO.0189-21.2021.

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Isolation and Preservation of Salmonella

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Following identification of the isolates recovered after sanitization, a loop of culture from Salmonella-positive triple sugar iron slant was streaked onto XLT4 plates and incubated at 37°C for 24 h. A distinct Salmonella colony was picked from the XLT4 plate and streaked onto tryptic soy agar (BD236950) and incubated. Afterward, cells were harvested and preserved in cryotubes containing a 20% glycerol solution (G33500, Fisher Scientific) and stored at −80°C.

Obe T., Nannapaneni R., Schilling W., Zhang L., McDaniel C, & Kiess A. (2020). Prevalence of Salmonella enterica on poultry processing equipment after completion of sanitization procedures. Poultry Science, 99(9), 4539-4548.

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