P44 42 mapk erk1 2

Manufactured by Abcam

Sourced in United Kingdom, Germany

P44/42 MAPK (ERK1/2) is a primary antibody that recognizes the extracellular signal-regulated kinase 1 and 2 (ERK1/2), also known as p44/42 MAPK. ERK1/2 are members of the mitogen-activated protein kinase (MAPK) family and play a central role in the MAPK/ERK signaling cascade.

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Lab products found in correlation

Phospho akt ser473, by Abcam (2 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204, by Abcam (2 mentions) Nf κb p65 phospho s536, by Abcam (1 mentions) Myd88, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Tumor necrosis factor alpha tnf α, by Cell Signaling Technology (1 mentions) Donkey anti rabbit alexa 488, by Thermo Fisher Scientific (1 mentions) Donkey anti goat alexa568, by Thermo Fisher Scientific (1 mentions) Proliferating cell nuclear antigen (pcna), by Merck Group (1 mentions) Iba 1, by Abcam (1 mentions) β actin, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

ERK1/2 (92 citations) P44/42 MAPK (2 citations) Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (2 citations) Anti-p44/42 MAPK (ERK1/2 (2 citations) Anti-phospho-p44/42 MAPK (ERK1/2 (2 citations)

2 protocols using p44 42 mapk erk1 2

Monoclonal Antibody Production for PI3Kγ Catalytic Subunit

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Monoclonal antibody against the catalytic subunit p110γ of PI3Kγ was produced in our laboratory (23 (link)). Other primary antibodies have been purchased from Cell Signaling (USA): tumor necrosis factor alpha (TNF-α) (#3707), phospho-NFκB p65 (S536) (#3033), phospho-Akt (Ser473) (#9271), Akt (#9272), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (#9106), p44/42 MAPK (ERK1/2) (#9107), MyD88 (#4283), and TLR4 (#14358).
Antibodies against inducible nitric oxide synthase (iNOS) was obtained from Abcam (UK, #ab3523) and against β-actin (#A2228 and #A5441) from Sigma-Aldrich (St. Louis, USA). Secondary HRP-coupled anti-rabbit and anti-mouse antibodies were purchased from KPL (Weden, Germany).

Lajqi T., Lang G.P., Haas F., Williams D.L., Hudalla H., Bauer M., Groth M., Wetzker R, & Bauer R. (2019). Memory-Like Inflammatory Responses of Microglia to Rising Doses of LPS: Key Role of PI3Kγ. Frontiers in Immunology, 10, 2492.

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Antibody Production and Characterization

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Monoclonal anti-mouse PI3Kγ antibody was produced in our facility [23 (link)]. Other antibodies were obtained from Cell Signaling Technologies (Danvers, MA, USA) (PCNA, #2586); Sigma Aldrich (Steinheim, Germany) (β actin, #A5441); Cell Signaling (Danvers, MA, USA) (phospho-Akt (Ser473), #9271; Akt, #9106; phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204), #9107; p44/42 MAPK (ERK1/2), #9107); and Abcam (Berlin, Germany) (Iba1 (#ab5076) and Ki67 (ab16667)). Secondary antibodies Alexa 488 donkey anti-rabbit (#A21206) and Alexa 568 donkey anti-goat (#A11057) were purchased from Invitrogen (Carlsbad, CA, USA), whereas HRP-coupled anti-rabbit and anti-mouse antibodies were purchased from KPL (Weden, Germany).

Schmidt C., Schneble-Löhnert N., Lajqi T., Wetzker R., Müller J.P, & Bauer R. (2021). PI3Kγ Mediates Microglial Proliferation and Cell Viability via ROS. Cells, 10(10), 2534.

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